| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | B220;CD 45;CD45;CD45 antigen;CD45R;GP180;L-CA;LCA;Leukocyte common antigen;loc;Ly-5;LY5;Ly5, homolog of;Lyt-4;OTTHUMP00000033813;OTTHUMP00000033816;OTTHUMP00000033817;OTTHUMP00000038574;Protein tyrosine phosphatase receptor type c polypeptide;Protein tyrosine phosphatase, receptor type C;protein tyrosine phosphatase, receptor type, C;Protein tyrosine phosphatase, receptor type, c polypeptide;Ptprc;PTPRC_HUMAN;Receptor-type tyrosine-protein phosphatase C;T200;T200 glycoprotein;T200 leukocyte common antigen |
| Antigen | |
| Cellular Localization | |
| Clonality | |
| Conjugate | |
| Formulation | |
| Gene ID | |
| Host | |
| Immunogen | Synthesized peptide derived from human CD45 (LCA) AA range: 500-600 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target |
Product Overview
U-Blot® CD45 (ABT-CD45) Mouse Monoclonal Antibody (WM1387) is a mouse monoclonal antibody from UcallM Biosciences Inc, raised against CD45. It has been validated for Immunohistochemistry, Western Blot, Immunofluorescence, ELISA and reacts with Human.
Validated Applications
| Target | CD45 |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | ABT-CD45 |
| Isotype | IgG2b, Kappa |
| Conjugate | Unconjugated |
| Reactivity | Human |
| Applications | Immunohistochemistry, Western Blot, Immunofluorescence, ELISA |
| Purification | Protein G |
| Specificity | The antibody can specifically recognize human CD45 protein, including CD45RA, CD45RB and CD45RO. |
| Molecular Weight | Observed: 200 kDa |
| UniProt / Swiss-Prot | P08575-3 (Human) |
Recommended dilutions: IHC 1:200-1000;WB 1:500-2000;IF 1:100-500;ELISA 1:1000-5000
Immunogen / Antigen
Synthesized peptide derived from human CD45 (LCA) AA range: 500-600
Reactivity Notes
Confirmed cross-reactivity: Human. Confirm suitability for untested species before use.
Scientific Background
CD45, also known as leukocyte common antigen, is a specific marker of hematopoietic cells. CD45 consists of at least four isoforms produced by alternative messenger RNA splicing. The isoforms share a common large intracellular domain and the extracellular domains are rod shaped, heavily glycosylated. It is expressed in all T cells, B cells and NK cells except plasma cells, monocytes, granulocytes and macrophages, but not in erythrocytes, platelets and non hematopoietic systems, Therefore, it can be used as a good marker to distinguish lymphoma or leukemia from non hematopoietic tumors.
Safety & Handling
This antibody is validated for Immunohistochemistry, Western Blot, Immunofluorescence, ELISA. Recommended dilutions: IHC 1:200-1000;WB 1:500-2000;IF 1:100-500;ELISA 1:1000-5000
Confirmed reactivity: Human. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Clone ABT-CD45.
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
