| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | CD 5;CD5;CD5 antigen;p56 62;CD5 antigen;CD5 molecule;CD5_HUMAN;LEU1;LyA;Lymphocyte antigen T1/Leu-1;OTTHUMP00000236973;T-cell surface glycoprotein CD5;T1 |
| Antigen | |
| Cellular Localization | |
| Clonality | |
| Conjugate | |
| Formulation | |
| Gene ID | |
| Host | |
| Immunogen | Synthesized peptide derived from human CD5 AA range: 200-300 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target |
Product Overview
U-Blot® CD5 (ABT-CD5) Mouse Monoclonal Antibody (WM1388) is a mouse monoclonal antibody from UcallM Biosciences Inc, raised against CD5. It has been validated for Immunohistochemistry, Western Blot, Immunofluorescence, ELISA and reacts with Human.
Validated Applications
| Target | CD5 |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | ABT-CD5 |
| Isotype | IgG2a, Kappa |
| Conjugate | Unconjugated |
| Reactivity | Human |
| Applications | Immunohistochemistry, Western Blot, Immunofluorescence, ELISA |
| Purification | Protein G |
| Specificity | The antibody can specifically recognize human CD5 protein. In western blotting of Jurkat cell lysate, the antibody can label a 55 kDa band corresponding to CD5. |
| Molecular Weight | Calculated: 55 kDa | Observed: 65 kDa |
| UniProt / Swiss-Prot | P06127 (Human) |
Recommended dilutions: IHC 1:200-1000;WB 1:500-2000;IF 1:100-500;ELISA 1:1000-5000
Immunogen / Antigen
Synthesized peptide derived from human CD5 AA range: 200-300
Reactivity Notes
Confirmed cross-reactivity: Human. Confirm suitability for untested species before use.
Scientific Background
CD5 is a 67 kDa transmembrane glycoprotein expressed on the surface of T cells, and its intensity increases with the maturation of T cells. CD5 is also expressed in B cells in normal human peripheral blood and lymph node mantle cells. CD5 is detected in most T-cell lymphomas and leukaemias. It is mainly used for the auxiliary diagnosis of T-cell leukemia and T-cell lymphoma. It can also be used to distinguish thymic cancer from other adenocarcinoma.
Safety & Handling
This antibody is validated for Immunohistochemistry, Western Blot, Immunofluorescence, ELISA. Recommended dilutions: IHC 1:200-1000;WB 1:500-2000;IF 1:100-500;ELISA 1:1000-5000
Confirmed reactivity: Human. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Clone ABT-CD5.
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
