| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Antigen identified by monoclonal BB2;BB 2;BB2;CD 54;CD_antigen=CD54;CD54;Cell surface glycoprotein P3.58;Human rhinovirus receptor;ICAM 1;ICAM-1;ICAM1;ICAM1_HUMAN;intercellular adhesion molecule 1;CD54;intercellular adhesion molecule 1;CD54;, human rhinovirus receptor;Intercellular adhesion molecule 1;Major group rhinovirus receptor;MALA 2;MALA2;MyD 10;MyD10;P3.58;Surface antigen of activated B cells;Surface antigen of activated B cells, BB2 |
| Antigen | |
| Cellular Localization | |
| Clonality | |
| Conjugate | |
| Formulation | |
| Gene ID | |
| Host | |
| Immunogen | Synthesized peptide derived from human CD54 AA range: 100-200 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target |
Product Overview
U-Blot® CD54 (ABT-CD54) Mouse Monoclonal Antibody (WM1389) is a mouse monoclonal antibody from UcallM Biosciences Inc, raised against ICAM-1. It has been validated for Immunohistochemistry, Western Blot, Immunofluorescence, ELISA and reacts with Human.
Validated Applications
| Target | ICAM-1 |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | ABT-CD54 |
| Isotype | IgG1, Kappa |
| Conjugate | Unconjugated |
| Reactivity | Human |
| Applications | Immunohistochemistry, Western Blot, Immunofluorescence, ELISA |
| Purification | Protein G |
| Specificity | The antibody can specifically recognize human CD54 protein. |
| Molecular Weight | Calculated: 58 kDa | Observed: 100 kDa |
| UniProt / Swiss-Prot | P05362 (Human) |
Recommended dilutions: IHC 1:200-1000;WB 1:500-2000;IF 1:100-500;ELISA 1:1000-5000
Immunogen / Antigen
Synthesized peptide derived from human CD54 AA range: 100-200
Reactivity Notes
Confirmed cross-reactivity: Human. Confirm suitability for untested species before use.
Scientific Background
CD54, also known as ICAM-1, is a type I transmembrane protein. CD is expressed on activated endothelial cells, high endothelial venules, monocytes/macrophages, T and B cells, dendritic cells and granulocytes. It plays a role in cellular adhesion and is involved in inflammation and leukocyte extravasation. CD54 is also detected in some carcinoma and melanoma cells. In patients with various inflammatory syndromes, the level of soluble CD54 is upregulated in plasma.
Safety & Handling
This antibody is validated for Immunohistochemistry, Western Blot, Immunofluorescence, ELISA. Recommended dilutions: IHC 1:200-1000;WB 1:500-2000;IF 1:100-500;ELISA 1:1000-5000
Confirmed reactivity: Human. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Clone ABT-CD54.
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
