| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | alpha polypeptide;p32;CD_antigen=CD8a;CD8;CD8 antigen alpha polypeptide;CD8 antigen alpha polypeptide;p32;CD8 antigen, alpha polypeptide;p32;CD8a;CD8A antigen;CD8A molecule;CD8A_HUMAN;Leu2;Leu2 T lymphocyte antigen;Ly 2;Ly 35;Ly B;Ly2;Ly3;Ly35;LyB;Lyt 2.1 lymphocyte differentiation antigen;AA at 100;LYT3;MAL;OKT8 T cell antigen;OTTHUMP00000160760;OTTHUMP00000160764;OTTHUMP00000203528;OTTHUMP00000203721;p32;T cell antigen Leu2;T cell co receptor;T lymphocyte differentiation antigen T8/Leu 2;T-cell surface glycoprotein CD8 alpha chain;T-cell surface glycoprotein Lyt 2;T-lymphocyte differentiation antigen T8/Leu-2;T8 T cell antigen;T8/Leu-2 T-lymphocyte differentiation antigen |
| Antigen | |
| Cellular Localization | |
| Clonality | |
| Conjugate | |
| Formulation | |
| Gene ID | |
| Host | |
| Immunogen | Synthesized peptide derived from human protein. AA range:100-235 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target |
Product Overview
U-Blot® CD8 a (ABT304) Mouse Monoclonal Antibody (WM1470) is a mouse monoclonal antibody from UcallM Biosciences Inc, raised against CD8. It has been validated for Immunohistochemistry, Western Blot, Immunofluorescence, ELISA and reacts with Human.
Validated Applications
| Target | CD8 |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | ABT304 |
| Isotype | IgG2b, Kappa |
| Conjugate | Unconjugated |
| Reactivity | Human |
| Applications | Immunohistochemistry, Western Blot, Immunofluorescence, ELISA |
| Purification | Protein G |
| Specificity | The antibody can specifically recognize human CD8 protein, including two typies of dimer: αβ heterodimer or αα homodimer. |
| Molecular Weight | Calculated: 26 kDa | Observed: 30-35 kDa |
| UniProt / Swiss-Prot | P01732 (Human) | P01731 (Mouse) |
Recommended dilutions: IHC 1:200-1000;WB 1:500-2000;IF 1:100-500;ELISA 1:1000-5000
Immunogen / Antigen
Synthesized peptide derived from human protein. AA range:100-235
Reactivity Notes
Confirmed cross-reactivity: Human. Confirm suitability for untested species before use.
Scientific Background
CD8 is a 68 kDa transmembrane glycoprotein consists of two subunits, αβ heterodimer or αα homodimer linked by a disulfide. CD8 is mainly expressed on the surface of suppress/cytotoxic T cells and NK cells. It is detected to be positive in T-cell large granular lymphocytic leukemia and subcutaneous panniculitis like T-cell lymphoma, and can be a marker for Precursor T lymphoblastic leukemia / lymphoma. CD8 is negative in Mycosis Fungoides and non-hematolymphoid neoplasms.
Safety & Handling
This antibody is validated for Immunohistochemistry, Western Blot, Immunofluorescence, ELISA. Recommended dilutions: IHC 1:200-1000;WB 1:500-2000;IF 1:100-500;ELISA 1:1000-5000
Confirmed reactivity: Human. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Clone ABT304.
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
