| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | 12E7;Antigen identified by monoclonal 12E7, Y homolog;Antigen identified by monoclonal antibodies 12E7, F21 and O13;CD99;Cell surface antigen 12E7;Cell surface antigen HBA 71;Cell surface antigen O13;E2 antigen;HBA71;MIC 2X;MIC 2Y;MIC2;MIC2X;MIC2Y;MSK5X;Protein MIC2;Surface antigen MIC2;T cell surface glycoprotein E2 |
| Antigen | |
| Cellular Localization | |
| Clonality | |
| Conjugate | |
| Formulation | |
| Gene ID | |
| Host | |
| Immunogen | Synthesized peptide derived from human CD99 AA range: 1-100 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target |
Product Overview
U-Blot® CD99 (ABT156) Mouse Monoclonal Antibody (WM1396) is a mouse monoclonal antibody from UcallM Biosciences Inc, raised against CD99. It has been validated for Immunohistochemistry, Western Blot, Immunofluorescence, ELISA and reacts with Human.
Validated Applications
| Target | CD99 |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | ABT156 |
| Isotype | IgG1, Kappa |
| Conjugate | Unconjugated |
| Reactivity | Human |
| Applications | Immunohistochemistry, Western Blot, Immunofluorescence, ELISA |
| Purification | Protein G |
| Specificity | The antibody can specifically recognize human CD99 protein. |
| Molecular Weight | Calculated: 19 kDa | Observed: 28 kDa |
| UniProt / Swiss-Prot | P14209 (Human) |
Recommended dilutions: IHC 1:200-1000;WB 1:500-2000;IF 1:100-500;ELISA 1:1000-5000
Immunogen / Antigen
Synthesized peptide derived from human CD99 AA range: 1-100
Reactivity Notes
Confirmed cross-reactivity: Human. Confirm suitability for untested species before use.
Scientific Background
CD99 is a 32 kDa transmembrane glycoprotein which is thought to be involved in cell adhesion processes. CD99 was expressed in lymphocytes, thymic cortical cells, ovarian granulosa cells, islet cells, Sertoli cells of testis, immature terminal deoxynucleotidyl transferase (TDT) positive T cells, some bone marrow precursor cells and fibroblasts. At the same time, CD99 was also expressed in vascular endothelial cells, urethral epithelium, some columnar and squamous epithelial cells and ependymal cells. It is mainly used for the diagnosis of Ewing's sarcoma and primitive peripheral nerve endodermal tumor.
Safety & Handling
This antibody is validated for Immunohistochemistry, Western Blot, Immunofluorescence, ELISA. Recommended dilutions: IHC 1:200-1000;WB 1:500-2000;IF 1:100-500;ELISA 1:1000-5000
Confirmed reactivity: Human. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Clone ABT156.
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
