| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | NOTCH1;TAN1;Neurogenic locus notch homolog protein 1;Notch 1;hN1;Translocation-associated notch protein TAN-1 |
| Antigen | |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Conjugate | |
| Formulation | |
| Gene ID | |
| Host | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Notch 1. AA range:1735-1784 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target |
Product Overview
U-Blot® Cleaved-Notch 1 (V1754) Rabbit Polyclonal Antibody is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against Notch-1. It has been validated for Western Blot, Immunofluorescence, Immunohistochemistry, ELISA and reacts with Human, Mouse, Rat.
Validated Applications
| Target | Notch-1 |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Applications | Western Blot, Immunofluorescence, Immunohistochemistry, ELISA |
| Purification | Affinity Purified |
| Specificity | Cleaved-Notch 1 (V1754) Polyclonal Antibody detects endogenous levels of fragment of activated Notch 1 protein resulting from cleavage adjacent to V1754. |
| Molecular Weight | Observed: 110 kDa |
| UniProt / Swiss-Prot | P46531 (Human) | Q01705 (Mouse) | Q07008 (Rat) |
Recommended dilutions: WB 1:500-2000;IHC 1:50-300;IF 1:50-300
Immunogen / Antigen
The antiserum was produced against synthesized peptide derived from human Notch 1. AA range:1735-1784
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse, Rat. Confirm suitability for untested species before use.
Scientific Background
notch 1(NOTCH1) Homo sapiens This gene encodes a member of the NOTCH family of proteins. Members of this Type I transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple different domain types. Notch signaling is an evolutionarily conserved intercellular signaling pathway that regulates interactions between physically adjacent cells through binding of Notch family receptors to their cognate ligands. The encoded preproprotein is proteolytically processed in the trans-Golgi network to generate two polypeptide chains that heterodimerize to form the mature cell-surface receptor. This receptor plays a role in the development of numerous cell and tissue types. Mutations in this gene are associated with aortic valve disease, Adams-Oliver syndrome, T-cell acute lymphoblastic leukemia, chronic lymph
Safety & Handling
This antibody is validated for Western Blot, Immunofluorescence, Immunohistochemistry, ELISA. Recommended dilutions: WB 1:500-2000;IHC 1:50-300;IF 1:50-300
Confirmed reactivity: Human, Mouse, Rat. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
