| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | PARP1;ADPRT;PPOL;Poly [ADP-ribose] polymerase 1;PARP-1;ADP-ribosyltransferase diphtheria toxin-like 1;ARTD1;NAD;+;ADP-ribosyltransferase 1;ADPRT 1;Poly[ADP-ribose] synthase 1 |
| Antigen | |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Conjugate | |
| Formulation | |
| Gene ID | |
| Host | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human PARP. AA range:165-214 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target |
Product Overview
U-Blot® Cleaved-PARP-1 (D214) Rabbit Polyclonal Antibody is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against PARP. It has been validated for Western Blot, Immunohistochemistry, Immunofluorescence, ELISA and reacts with Human, Mouse.
Validated Applications
| Target | PARP |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse |
| Applications | Western Blot, Immunohistochemistry, Immunofluorescence, ELISA |
| Purification | Affinity Purified |
| Specificity | Cleaved-PARP-1 (D214) Polyclonal Antibody detects endogenous levels of fragment of activated PARP-1 protein resulting from cleavage adjacent to D214. |
| Molecular Weight | Observed: 24 kDa |
| UniProt / Swiss-Prot | P09874 (Human) | P11103 (Mouse) |
Recommended dilutions: WB 1:500-2000;IF 1:50-300;IHC 1:50-300
Immunogen / Antigen
The antiserum was produced against synthesized peptide derived from human PARP. AA range:165-214
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse. Confirm suitability for untested species before use.
Scientific Background
This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes. [provided by RefSeq, Jul 2008]
Safety & Handling
This antibody is validated for Western Blot, Immunohistochemistry, Immunofluorescence, ELISA. Recommended dilutions: WB 1:500-2000;IF 1:50-300;IHC 1:50-300
Confirmed reactivity: Human, Mouse. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
