| Field | Specification |
|---|---|
| Alternative Names | CTTN;EMS1;Src substrate cortactin;Amplaxin;Oncogene EMS1 |
| Antigen | |
| Cellular Localization | |
| Clonality | |
| Conjugate | |
| Formulation | |
| Gene ID | |
| Host | |
| Immunogen | Purified recombinant fragment of human Cortactin expressed in E. Coli. |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target |
Product Overview
U-Blot® Cortactin Mouse Monoclonal Antibody is a mouse monoclonal antibody from UcallM Biosciences Inc, raised against Cortactin. It has been validated for Western Blot, Immunohistochemistry, Immunofluorescence, ELISA and reacts with Human, Mouse.
Validated Applications
| Target | Cortactin |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse |
| Applications | Western Blot, Immunohistochemistry, Immunofluorescence, ELISA |
| Purification | Affinity Purified |
| Specificity | Cortactin Monoclonal Antibody detects endogenous levels of Cortactin protein. |
| Molecular Weight | Calculated: 62 kDa |
| UniProt / Swiss-Prot | Q14247 (Human) | Q60598 (Mouse) |
Recommended dilutions: WB 1:500-2000;IHC 1:200-400;IF/ICC1:200-400;ELISA 1:5000-20000
Immunogen / Antigen
Purified recombinant fragment of human Cortactin expressed in E. Coli.
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse. Confirm suitability for untested species before use.
Scientific Background
cortactin(CTTN) Homo sapiens This gene is overexpressed in breast cancer and squamous cell carcinomas of the head and neck. The encoded protein is localized in the cytoplasm and in areas of the cell-substratum contacts. This gene has two roles: (1) regulating the interactions between components of adherens-type junctions and (2) organizing the cytoskeleton and cell adhesion structures of epithelia and carcinoma cells. During apoptosis, the encoded protein is degraded in a caspase-dependent manner. The aberrant regulation of this gene contributes to tumor cell invasion and metastasis. Three splice variants that encode different isoforms have been identified for this gene. [provided by RefSeq, May 2010]
Safety & Handling
This antibody is validated for Western Blot, Immunohistochemistry, Immunofluorescence, ELISA. Recommended dilutions: WB 1:500-2000;IHC 1:200-400;IF/ICC1:200-400;ELISA 1:5000-20000
Confirmed reactivity: Human, Mouse. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
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