| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | CK 14;CK-14;ck14;Cytokeratin 14;CytokeRatin-14;CytokeRatin14;Dowling Meara;EBS3;EBS4;Epidermolysis bullosa simplex;K14;K1C14_HUMAN;KeRatin 14;epidermolysis bullosa simplex, Dowling-Meara, Koebner;KeRatin 14;KeRatin;KeRatin type I cytoskeletal 14;KeRatin, type I cytoskeletal 14;KeRatin-14;KeRatin14;Koebner;Krt 14;Krt14;NFJ;OTTHUMP00000164624;type I cytoskeletal 14 |
| Antigen | |
| Cellular Localization | |
| Clonality | |
| Conjugate | |
| Formulation | |
| Gene ID | |
| Host | |
| Immunogen | Synthesized peptide derived from human Cytokeratin 14 AA range: 400-472 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target |
Product Overview
U-Blot® Cytokeratin 14 (PT1674) Mouse Monoclonal Antibody (WM1472) is a mouse monoclonal antibody from UcallM Biosciences Inc, raised against Cytokeratin 14. It has been validated for Immunohistochemistry, Western Blot, Immunofluorescence, ELISA and reacts with Human, Mouse, Rat.
Validated Applications
| Target | Cytokeratin 14 |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | PT1674 |
| Isotype | IgG1, Kappa |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Applications | Immunohistochemistry, Western Blot, Immunofluorescence, ELISA |
| Purification | Protein G |
| Specificity | The antibody can specifically recognize human CK14 protein. In immunohistochemistry on formalin-fixed, paraffin-embedded tissue sections, the antibody specifically labels the basal cell of squamous epithelial cells and glandular epithelia, myoepithelium and mesothelial cells. |
| Molecular Weight | Calculated: 52 kDa | Observed: 53 kDa |
| UniProt / Swiss-Prot | P02533 (Human) |
Recommended dilutions: IHC 1:200-1000;WB 1:500-2000;IF 1:100-500;ELISA 1:1000-5000
Immunogen / Antigen
Synthesized peptide derived from human Cytokeratin 14 AA range: 400-472
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse, Rat. Confirm suitability for untested species before use.
Scientific Background
CK14 is a member of the Type I family of cytokeratins with a molecular weight of 50 kDa, which is expressed in the basal cells of myoepithelium and keratinized squamous epithelium. It is mainly used for the difference between stratified squamous epithelium and monolayer epithelium and the diagnosis of squamous cell carcinoma. Eosinophilic tumors at multiple anatomical sites express CK14, which can be used to distinguish from adenocarcinoma, invasive ductal carcinoma of salivary gland and intraductal carcinoma.
Safety & Handling
This antibody is validated for Immunohistochemistry, Western Blot, Immunofluorescence, ELISA. Recommended dilutions: IHC 1:200-1000;WB 1:500-2000;IF 1:100-500;ELISA 1:1000-5000
Confirmed reactivity: Human, Mouse, Rat. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Clone PT1674.
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
