| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | CK 18;CK-18;CK18;CYK18;Cytokeratin 18;Cytokeratin endo B;CytokeRatin-18;K 18;K18;K1C18_HUMAN;KA18;KeRatin 18;KeRatin 18, type I;KeRatin D;keRatin, type I cytoskeletal 18;KeRatin-18;Krt18 |
| Antigen | |
| Cellular Localization | |
| Clonality | |
| Conjugate | |
| Formulation | |
| Gene ID | |
| Host | |
| Immunogen | Synthesized peptide derived from human Cytokeratin 18 AA range: 200-300 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target |
Product Overview
U-Blot® Cytokeratin 18 (ABT054) Mouse Monoclonal Antibody (WM1401) is a mouse monoclonal antibody from UcallM Biosciences Inc, raised against Cytokeratin 18. It has been validated for Immunohistochemistry, Western Blot, Immunofluorescence, ELISA and reacts with Human, Mouse, Rat.
Validated Applications
| Target | Cytokeratin 18 |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | ABT054 |
| Isotype | IgG2b, Kappa |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Applications | Immunohistochemistry, Western Blot, Immunofluorescence, ELISA |
| Purification | Protein G |
| Specificity | The antibody can specifically recognize human CK18 protein, and shows no cross reaction with CK1, 5, 6, 7, 8, 10, 13, 14, 15, 19, 20. In western blotting of Hela and MCF7 cell lysates, the antibody can label a 45 kDa band corresponding to CK18. |
| Molecular Weight | Calculated: 48 kDa | Observed: 48 kDa |
| UniProt / Swiss-Prot | P05783 (Human) |
Recommended dilutions: IHC 1:200-1000;WB 1:500-2000;IF 1:100-500;ELISA 1:1000-5000
Immunogen / Antigen
Synthesized peptide derived from human Cytokeratin 18 AA range: 200-300
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse, Rat. Confirm suitability for untested species before use.
Scientific Background
CK18 is a cytokeratin with a molecular weight of 45 kDa. It is mainly expressed in monolayer and pseudostratified epithelium, but negative in stratified squamous epithelium. It is expressed in glandular epithelium, transitional epithelium and hepatocytes. It is mainly used for the diagnosis of adenocarcinoma.
Safety & Handling
This antibody is validated for Immunohistochemistry, Western Blot, Immunofluorescence, ELISA. Recommended dilutions: IHC 1:200-1000;WB 1:500-2000;IF 1:100-500;ELISA 1:1000-5000
Confirmed reactivity: Human, Mouse, Rat. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Clone ABT054.
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
