| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | 40 kDa keRatin intermediate filament;CK 19;CK-19;CK19;Cytokeratin 19;CytokeRatin-19;K19;K1C19_HUMAN;K1CS;KeRatin 19;KeRatin type I 40 kD;KeRatin type I 40kD;KeRatin type I cytoskeletal 19;KeRatin, type I cytoskeletal 19;KeRatin, type I, 40 kd;KeRatin-19;KRT19;MGC15366 |
| Antigen | |
| Cellular Localization | |
| Clonality | |
| Conjugate | |
| Formulation | |
| Gene ID | |
| Host | |
| Immunogen | Synthesized peptide derived from human Cytokeratin 19 AA range: 200-300 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target |
Product Overview
U-Blot® Cytokeratin 19&10 (ABT-CK19) Mouse Monoclonal Antibody is a mouse monoclonal antibody from UcallM Biosciences Inc, raised against Cytokeratin 19/10. It has been validated for Immunohistochemistry, Western Blot, Immunofluorescence, ELISA and reacts with Human.
Validated Applications
| Target | Cytokeratin 19/10 |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | ABT-CK19 |
| Isotype | IgG1, Kappa |
| Conjugate | Unconjugated |
| Reactivity | Human |
| Applications | Immunohistochemistry, Western Blot, Immunofluorescence, ELISA |
| Purification | Protein G |
| Specificity | The antibody can recognize human CK10 and CK19 protein, and shows no cross reaction with CK1, 5, 6, 7, 8, 13, 14, 15, 17, 18, 20. |
| Molecular Weight | Calculated: 44 kDa | Observed: 44 kDa |
| UniProt / Swiss-Prot | P08727;P13645 (Human) |
Recommended dilutions: IHC 1:200-1000;WB 1:500-2000;IF 1:100-500;ELISA 1:1000-5000
Immunogen / Antigen
Synthesized peptide derived from human Cytokeratin 19 AA range: 200-300
Reactivity Notes
Confirmed cross-reactivity: Human. Confirm suitability for untested species before use.
Scientific Background
CK19 is the smallest keratin with a molecular weight of 40-44 kDa. which is widely distributed in monolayer epithelial cells and many squamous epithelial tissues, and plays an important role in maintaining cell structure and morphology. Its function is related to cell proliferation, differentiation, invasion and metastasis, and cell cycle, etc. CK19 is an important biomarker of breast cancer cells, lymph nodes, peripheral blood and bone marrow tumor cells. The keratin of CK10 with molecular weight of about 60kD is not expressed in epidermal basal cells under normal conditions. When the cells generated by basal cell division undergo neoplasia, CK1 / CK10 begins to be expressed. The expression of CK10 is correlated with the differentiation degree of malignant keratinocytes, and the higher the positive expression in tissues, the stronger the differentiation represented by CK10.
Safety & Handling
This antibody is validated for Immunohistochemistry, Western Blot, Immunofluorescence, ELISA. Recommended dilutions: IHC 1:200-1000;WB 1:500-2000;IF 1:100-500;ELISA 1:1000-5000
Confirmed reactivity: Human. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Clone ABT-CK19.
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
