| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | CD20;CK 20;CK-20;CK20;CytokeRatin-20;CytokeRatin20;K1C20_HUMAN;K20;KA20;KeRatin 20;keRatin 20, type I;keRatin 21, Rat, homolog of;KeRatin;KeRatin type I cytoskeletal 20;KeRatin-20;KeRatin20;KRT 20;KRT 21;KRT20;KRT21;MGC35423;OTTHUMP00000164518;Protein IT;type I cytoskeletal 20 |
| Antigen | |
| Cellular Localization | |
| Clonality | |
| Conjugate | |
| Formulation | |
| Gene ID | |
| Host | |
| Immunogen | Synthesized peptide derived from human CK20 AA range: 300-424 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target |
Product Overview
U-Blot® Cytokeratin 20 (ABT044) Mouse Monoclonal Antibody (WM1404) is a mouse monoclonal antibody from UcallM Biosciences Inc, raised against Cytokeratin 20. It has been validated for Immunohistochemistry, Western Blot, Immunofluorescence, ELISA and reacts with Human, Mouse, Rat.
Validated Applications
| Target | Cytokeratin 20 |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | ABT044 |
| Isotype | IgG2b, Kappa |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Applications | Immunohistochemistry, Western Blot, Immunofluorescence, ELISA |
| Purification | Protein G |
| Specificity | The antibody can specifically recognize human CK20 protein, and shows no cross reaction with CK1, 5, 6, 7, 8, 13, 14, 15, 17, 18. |
| Molecular Weight | Calculated: 48 kDa | Observed: 48 kDa |
| UniProt / Swiss-Prot | P35900 (Human) |
Recommended dilutions: IHC 1:200-1000;WB 1:500-2000;IF 1:100-500;ELISA 1:1000-5000
Immunogen / Antigen
Synthesized peptide derived from human CK20 AA range: 300-424
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse, Rat. Confirm suitability for untested species before use.
Scientific Background
CK20 is a cytokeratin with a molecular weight of 46 kDa, which is mainly expressed in gastrointestinal epithelium, urethral epithelium and Merkel cells. It is mainly used for the diagnosis of gastrointestinal adenocarcinoma, ovarian mucinous tumor, Merkel cell carcinoma and cholangiopancreatic adenocarcinoma. CK20 was negative in squamous cell carcinoma, lung adenocarcinoma, endometrial adenocarcinoma, breast cancer, small cell lung cancer and ovarian non mucinous tumor. It is often used in combination with CK7 and villin to judge the tissue source of adenocarcinoma.
Safety & Handling
This antibody is validated for Immunohistochemistry, Western Blot, Immunofluorescence, ELISA. Recommended dilutions: IHC 1:200-1000;WB 1:500-2000;IF 1:100-500;ELISA 1:1000-5000
Confirmed reactivity: Human, Mouse, Rat. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Clone ABT044.
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
