| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | CK 4;CK-4;CK4;CYK4;Cytokeratin 4;CytokeRatin-4;CytokeRatin4;FLJ31692;K2C4_HUMAN;K4;KeRatin 4;KeRatin;KeRatin type II cytoskeletal 4;KeRatin-4;KeRatin4;KRT 4;Krt4;type II cytoskeletal 4;Type-II keRatin Kb4 |
| Antigen | |
| Cellular Localization | |
| Clonality | |
| Conjugate | |
| Formulation | |
| Host | |
| Immunogen | Synthesized peptide derived from human Cytokeratin 4 AA range: 400-534 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target |
Product Overview
U-Blot® Cytokeratin 4 (ABT061) Mouse Monoclonal Antibody is a mouse monoclonal antibody from UcallM Biosciences Inc, raised against Cytokeratin 4. It has been validated for Immunohistochemistry, Western Blot, Immunofluorescence, ELISA and reacts with Human.
Validated Applications
| Target | Cytokeratin 4 |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | ABT061 |
| Isotype | IgG1, Kappa |
| Conjugate | Unconjugated |
| Reactivity | Human |
| Applications | Immunohistochemistry, Western Blot, Immunofluorescence, ELISA |
| Purification | Protein G |
| Specificity | The antibody can specifically recognize human CK4 protein, and shows no cross reaction with CK5, 6, 7, 8, 14, 15, 17, 20. |
| Molecular Weight | Calculated: 57 kDa | Observed: 57 kDa |
| UniProt / Swiss-Prot | P19013 (Human) |
Recommended dilutions: IHC 1:200-1000;WB 1:500-2000;IF 1:100-500;ELISA 1:1000-5000
Immunogen / Antigen
Synthesized peptide derived from human Cytokeratin 4 AA range: 400-534
Reactivity Notes
Confirmed cross-reactivity: Human. Confirm suitability for untested species before use.
Scientific Background
CK4 is a cytokeratin with a molecular weight of 59 kDa. It is a member of type II cytokeratin family. CK4 is widely expressed in non keratinocytes and stratified squamous epithelium. It is mainly used to label esophageal and oral squamous epithelial cells and tumors of their origin.
Safety & Handling
This antibody is validated for Immunohistochemistry, Western Blot, Immunofluorescence, ELISA. Recommended dilutions: IHC 1:200-1000;WB 1:500-2000;IF 1:100-500;ELISA 1:1000-5000
Confirmed reactivity: Human. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Clone ABT061.
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
