| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | 58 kDa cytokeratin;CK-5;CK5;Cytokeratin-5;Cytokeratin5;DDD;DDD1;EBS2;epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne types;K2C5_HUMAN;K5;keratin 5;epidermolysis bullosa simplex, Dowling-Meara/Kobner/Weber-Cockayne types;Keratin 5;Keratin;keratin complex 2, basic, gene 5;keratin, type II cytoskeletal 5;Keratin-5;Keratin5;KRT 5;Krt5;KRT5A;type II cytoskeletal 5;Type-II keratin Kb5;CK 6A;CK 6B;CK 6C;CK 6D;CK 6E;CK-6B;CK-6C;CK-6E;Cytokeratin 6a;Cytokeratin 6B;Cytokeratin 6C;Cytokeratin 6D;Cytokeratin 6E;Cytokeratin-6B;Cytokeratin-6C;Cytokeratin-6E;K2C6C_HUMAN;K6a keratin;K6b keratin;K6C;K6c keratin;K6d keratin;K6e keratin;Keratin;Keratin K6h;Keratin type II cytoskeletal 6A;Keratin type II cytoskeletal 6B;Keratin type II cytoskeletal 6C;Keratin type II cytoskeletal 6D;Keratin type II cytoskeletal 6E;Keratin-6C;KRT6A;KRT6B;KRT6C;KRT6D;KRT6E;type II cytoskeletal 6C;Type-II keratin Kb12;58 kDa Cytokeratin antibody;CK-5 antibody;CK5 antibody;Cytokeratin-5 antibody;Cytokeratin5 antibody;DDD antibody;DDD1 antibody;EBS2 antibody;epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne types antibody;K2C5_HUMAN antibody;K5 antibody;keratin 5;epidermolysis bullosa simplex, Dowling-Meara/Kobner/Weber-Cockayne types;antibody;Keratin 5 antibody;Keratin antibody;keratin complex 2, basic, gene 5 antibody;keratin, type II cytoskeletal 5 antibody;Keratin-5 antibody;Keratin5 antibody;KRT 5 antibody;Krt5 antibody;KRT5A antibody;type II cytoskeletal 5 antibody;Type-II keratin Kb5 antibody |
| Antigen | |
| Cellular Localization | |
| Clonality | |
| Conjugate | |
| Formulation | |
| Gene ID | |
| Host | |
| Immunogen | Synthesized peptide derived from human Cytokeratin 5 and Cytokeratin 6 AA range: C terminal amino acids 500-590 of Cytokeratin 5 and N terminal amino acids 2-100 of Cytokeratin 6 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target |
Product Overview
U-Blot® Cytokeratin 5/6 (ABT-CK5/052) Mouse Monoclonal Antibody (WM1407) is a mouse monoclonal antibody from UcallM Biosciences Inc, raised against Cytokeratin 5/6. It has been validated for Immunohistochemistry, Western Blot, Immunofluorescence, ELISA and reacts with Human.
Validated Applications
| Target | Cytokeratin 5/6 |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | ABT-CK5/052 |
| Isotype | IgG2a, Kappa |
| Conjugate | Unconjugated |
| Reactivity | Human |
| Applications | Immunohistochemistry, Western Blot, Immunofluorescence, ELISA |
| Purification | Protein G |
| Specificity | The antibody can recognize both human CK5 and CK6 protein, and shows no cross reaction with other typies of cytokeratin. In western blotting of A431 cell lysate, there shows a 56 kDa band dectected by the antibody. |
| Molecular Weight | Observed: 60,62 kDa |
| UniProt / Swiss-Prot | P13647;P02538;P04259;P48668 (Human) |
Recommended dilutions: IHC 1:200-1000;WB 1:500-2000;IF 1:100-500;ELISA 1:1000-5000
Immunogen / Antigen
Synthesized peptide derived from human Cytokeratin 5 and Cytokeratin 6 AA range: C terminal amino acids 500-590 of Cytokeratin 5 and N terminal amino acids 2-100 of Cytokeratin 6
Reactivity Notes
Confirmed cross-reactivity: Human. Confirm suitability for untested species before use.
Scientific Background
Cytokeratin 5/6 are high molecular weight keratins, which are normally expressed in squamous epithelium, basal cells of ductal epithelium, mesothelial cells, myoepithelium and basal cells of prostate, but not in glandular epithelium. Most adenocarcinoma are negative, and some adenocarcinoma can be weakly positive. The antibody is mainly used for the differential diagnosis of mesothelioma and adenocarcinoma, as well as the study of benign and malignant tumors of epithelial origin.
Safety & Handling
This antibody is validated for Immunohistochemistry, Western Blot, Immunofluorescence, ELISA. Recommended dilutions: IHC 1:200-1000;WB 1:500-2000;IF 1:100-500;ELISA 1:1000-5000
Confirmed reactivity: Human. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Clone ABT-CK5/052.
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
