| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | 58 kDa cytokeRatin;CK-5;CK5;CytokeRatin-5;CytokeRatin5;DDD;DDD1;EBS2;epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne types;K2C5_HUMAN;K5;keRatin 5;epidermolysis bullosa simplex, Dowling-Meara/Kobner/Weber-Cockayne types;KeRatin 5;KeRatin;keRatin complex 2, basic, gene 5;keRatin, type II cytoskeletal 5;KeRatin-5;KeRatin5;KRT 5;Krt5;KRT5A;type II cytoskeletal 5;Type-II keRatin Kb5 |
| Antigen | |
| Cellular Localization | |
| Clonality | |
| Conjugate | |
| Formulation | |
| Gene ID | |
| Host | |
| Immunogen | Synthesized peptide derived from human Cytokeratin 5 AA range: 500-590 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target |
Product Overview
U-Blot® Cytokeratin 5 (ABT051) Mouse Monoclonal Antibody (WM1475) is a mouse monoclonal antibody from UcallM Biosciences Inc, raised against Cytokeratin 5. It has been validated for Immunohistochemistry, Western Blot, Immunofluorescence, ELISA and reacts with Human, Dog.
Validated Applications
| Target | Cytokeratin 5 |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | ABT051 |
| Isotype | IgG2a, Kappa |
| Conjugate | Unconjugated |
| Reactivity | Human, Dog |
| Applications | Immunohistochemistry, Western Blot, Immunofluorescence, ELISA |
| Purification | Protein G |
| Specificity | The antibody can specifically recognize human CK5 protein, and shows no cross reaction with CK6. In western blotting of A431, A549 and Hela cell lysates, the antibody can label a 58 kDa band corresponding to CK5 only in A431 cell lysates. |
| Molecular Weight | Calculated: 62 kDa | Observed: 62 kDa |
| UniProt / Swiss-Prot | P13647 (Human) |
Recommended dilutions: IHC 1:200-1000;WB 1:500-2000;IF 1:100-500;ELISA 1:1000-5000
Immunogen / Antigen
Synthesized peptide derived from human Cytokeratin 5 AA range: 500-590
Reactivity Notes
Confirmed cross-reactivity: Human, Dog. Confirm suitability for untested species before use.
Scientific Background
CK5 is a cytokeratin with a molecular weight of 58 kDa. In normal tissues, CK5 is expressed in squamous epithelium, germinal layer cells of some squamous epithelium, basal cells of ductal epithelium, myoepithelial cells and mesothelial cells, and almost no expression in most glandular epithelial cells. Therefore, it can be used for the differential diagnosis of squamous cell carcinoma and adenocarcinoma, mesothelioma and adenocarcinoma, as well as benign and malignant tumors of prostate, breast and salivary gland epithelium.
Safety & Handling
This antibody is validated for Immunohistochemistry, Western Blot, Immunofluorescence, ELISA. Recommended dilutions: IHC 1:200-1000;WB 1:500-2000;IF 1:100-500;ELISA 1:1000-5000
Confirmed reactivity: Human, Dog. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Clone ABT051.
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
