| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | CK 7;CK-7;CK7;Cytokeratin 7;CytokeRatin-7;D15Wsu77e;K2C7;K2C7_HUMAN;K7;KeRatin 7;KeRatin 7, type II;KeRatin type II cytoskeletal 7;KeRatin, 55K type II cytoskeletal;KeRatin, simple epithelial;KeRatin, simple epithelial type I, K7;KeRatin, type II cytoskeletal 7;KeRatin-7;Krt2-7;KRT7;MGC11625;MGC129731;MGC3625;Sarcolectin;SCL;Type II mesothelial keRatin K7;Type-II keRatin Kb7 |
| Antigen | |
| Cellular Localization | |
| Clonality | |
| Conjugate | |
| Formulation | |
| Gene ID | |
| Host | |
| Immunogen | Synthesized peptide derived from human Cytokeratin 7 AA range: 350-469 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target |
Product Overview
U-Blot® Cytokeratin 7 (ABT-CK7) Mouse Monoclonal Antibody (WM1408) is a mouse monoclonal antibody from UcallM Biosciences Inc, raised against Cytokeratin 7. It has been validated for Immunohistochemistry, Western Blot, Immunofluorescence, ELISA and reacts with Human.
Validated Applications
| Target | Cytokeratin 7 |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | ABT-CK7 |
| Isotype | IgG2b, Kappa |
| Conjugate | Unconjugated |
| Reactivity | Human |
| Applications | Immunohistochemistry, Western Blot, Immunofluorescence, ELISA |
| Purification | Protein G |
| Specificity | The antibody can specifically recognize human CK7 protein, and shows no cross reaction with CK1, 4, 5, 6, 8, 10, 14, 17, 18, 19, 20. In western blotting of Hela, A431, A549 and Hela cell lysates, the antibody can label a 51 kDa band corresponding to CK7 in A549 and Hela cell lysates, while there is no band detected in CK7 knock out Hela cell lysate. |
| Molecular Weight | Calculated: 51 kDa | Observed: 51 kDa |
| UniProt / Swiss-Prot | P08729 (Human) | Q9DCV7 (Mouse) | Q6IG12 (Rat) |
Recommended dilutions: IHC 1:200-1000;WB 1:500-2000;IF 1:100-500;ELISA 1:1000-5000
Immunogen / Antigen
Synthesized peptide derived from human Cytokeratin 7 AA range: 350-469
Reactivity Notes
Confirmed cross-reactivity: Human. Confirm suitability for untested species before use.
Scientific Background
CK7 is a cytokeratin with a molecular weight of 54 kDa, which mainly marks glandular epithelium and transitional epithelium. It was positive in the epithelium of breast, thyroid, ovary, endometrium and lung, but negative in the epithelium of gastrointestinal tract, colon and prostate. It is mainly used for the study of epithelial tumors. It is often used in combination with CK20 for differential diagnosis of gastrointestinal adenocarcinoma, lung adenocarcinoma and breast cancer, and combined with EMA for diagnosis of synovial sarcoma.
Safety & Handling
This antibody is validated for Immunohistochemistry, Western Blot, Immunofluorescence, ELISA. Recommended dilutions: IHC 1:200-1000;WB 1:500-2000;IF 1:100-500;ELISA 1:1000-5000
Confirmed reactivity: Human. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Clone ABT-CK7.
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
