| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | CARD2;CK 8;CK-8;CK8;CYK8;CYKER;Cytokeratin endo A;CytokeRatin-8;DreK8;EndoA;K2C8;K2C8_HUMAN;K8;KeRatin 8;KeRatin type II cytoskeletal 8;KeRatin, type II cytoskeletal 8;KeRatin-8;KO;Krt 2.8;KRT8;MGC118110;MGC174782;MGC53564;MGC85764;sb:cb186;Type-II keRatin Kb8 |
| Antigen | |
| Cellular Localization | |
| Clonality | |
| Conjugate | |
| Formulation | |
| Gene ID | |
| Host | |
| Immunogen | Synthesized peptide derived from human Cytokeratin 8 AA range: 400-483 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target |
Product Overview
U-Blot® Cytokeratin 8 (ABT535) Mouse Monoclonal Antibody (WM1409) is a mouse monoclonal antibody from UcallM Biosciences Inc, raised against Cytokeratin 8. It has been validated for Immunohistochemistry, Western Blot, Immunofluorescence, ELISA and reacts with Human, Mouse, Rat.
Validated Applications
| Target | Cytokeratin 8 |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | ABT535 |
| Isotype | IgG2a, Kappa |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Applications | Immunohistochemistry, Western Blot, Immunofluorescence, ELISA |
| Purification | Protein G |
| Specificity | The antibody can specifically recognize human CK8 protein, and shows no cross reaction with CK1, 4, 5, 6, 7, 10, 15, 16, 17, 18, 20. |
| Molecular Weight | Calculated: 54 kDa | Observed: 54 kDa |
| UniProt / Swiss-Prot | P05787 (Human) | P11679 (Mouse) | Q10758 (Rat) |
Recommended dilutions: IHC 1:200-1000;WB 1:500-2000;IF 1:100-500;ELISA 1:1000-5000
Immunogen / Antigen
Synthesized peptide derived from human Cytokeratin 8 AA range: 400-483
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse, Rat. Confirm suitability for untested species before use.
Scientific Background
Cytokeratin 8 (CK8) is a member of the type II family of cytokeratins with a molecular weight of 53 kDa which plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. It generally exists in monolayer and pseudostratified epithelium, and is expressed in glandular epithelium, transitional epithelium and hepatocytes. It is absent in squamous cell carcinomas. CK8 dimerizes with CK18 to form an intermediate filament in simple single-layered epithelial cells. CK8/18 expression patterns are useful for classification of tumors of unknown origin and poorly differentiated carcinomas.
Safety & Handling
This antibody is validated for Immunohistochemistry, Western Blot, Immunofluorescence, ELISA. Recommended dilutions: IHC 1:200-1000;WB 1:500-2000;IF 1:100-500;ELISA 1:1000-5000
Confirmed reactivity: Human, Mouse, Rat. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Clone ABT535.
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
