| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | CARD2;CK 8;CK-8;CK8;CYK8;CYKER;Cytokeratin endo A;CytokeRatin-8;DreK8;EndoA;K2C8;K2C8_HUMAN;K8;KeRatin 8;KeRatin type II cytoskeletal 8;KeRatin, type II cytoskeletal 8;KeRatin-8;KO;Krt 2.8;KRT8;MGC118110;MGC174782;MGC53564;MGC85764;sb:cb186;Type-II keRatin Kb8;CK 18;CK-18;CK18;CYK18;Cytokeratin 18;Cytokeratin endo B;CytokeRatin-18;K 18;K18;K1C18_HUMAN;KA18;KeRatin 18;KeRatin 18, type I;KeRatin D;keRatin, type I cytoskeletal 18;KeRatin-18;Krt18 |
| Cellular Localization | |
| Clonality | |
| Conjugate | |
| Formulation | |
| Gene ID | |
| Host | |
| Immunogen | Synthesized peptide derived from human Cytokeratin 8&18 AA range: C-term to CK8 and 200-300 to CK18 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target |
Product Overview
U-Blot® Cytokeratin 8&18 (ABT053/CK18) Mouse Monoclonal Antibody (WM1402) is a mouse monoclonal antibody from UcallM Biosciences Inc, raised against Cytokeratin 8/18. It has been validated for Immunohistochemistry, Western Blot, Immunofluorescence, ELISA and reacts with Human, Mouse, Rat.
Validated Applications
| Target | Cytokeratin 8/18 |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | ABT053/CK18 |
| Isotype | IgG2b, Kappa |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Applications | Immunohistochemistry, Western Blot, Immunofluorescence, ELISA |
| Purification | Protein G |
| Specificity | This product can specifically recognize human CK8 and CK18 protein. It is a cocktail of anti-CK8 and anti-CK18 monoclonal antibodies. |
| Molecular Weight | Observed: 48,54 kDa |
| UniProt / Swiss-Prot | P05787;P05783 (Human) |
Recommended dilutions: IHC 1:200-1000;WB 1:500-2000;IF 1:100-500;ELISA 1:1000-5000
Immunogen / Antigen
Synthesized peptide derived from human Cytokeratin 8&18 AA range: C-term to CK8 and 200-300 to CK18
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse, Rat. Confirm suitability for untested species before use.
Scientific Background
Cytokeratin 18 (CK18), an intermediated filament protein with a molecular weight of 45 KD, belongs to type I cytokeratin family. Cytokeratin 8 (CK8) is an intermediated filament protein with a molecular weight of 52 KD. It is a member of cytokeratin type II family. They are widely expressed in monolayer epithelial cells of thyroid, breast, gastrointestinal tract and respiratory tract. Ck8/18 is often used as a useful marker to identify adenocarcinoma from simple epithelial cells.
Safety & Handling
This antibody is validated for Immunohistochemistry, Western Blot, Immunofluorescence, ELISA. Recommended dilutions: IHC 1:200-1000;WB 1:500-2000;IF 1:100-500;ELISA 1:1000-5000
Confirmed reactivity: Human, Mouse, Rat. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Clone ABT053/CK18.
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
