| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | pan cytokeRatin;wide spectrum CytokeRatin;AE1;AE3 |
| Antigen | |
| Cellular Localization | |
| Clonality | |
| Conjugate | |
| Formulation | |
| Host | |
| Immunogen | Synthesized peptide derived from human Cytokeratin Pan AA range:100-200 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target |
Product Overview
U-Blot® Cytokeratin (ABT154) Mouse Monoclonal Antibody (WM1464) is a mouse monoclonal antibody from UcallM Biosciences Inc, raised against Cytokeratin Pan. It has been validated for Immunohistochemistry, Western Blot, Immunofluorescence, ELISA and reacts with Human, Mouse, Rat.
Validated Applications
| Target | Cytokeratin Pan |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | ABT154 |
| Isotype | IgG1, Kappa |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Applications | Immunohistochemistry, Western Blot, Immunofluorescence, ELISA |
| Purification | Protein G |
| Specificity | The antibody can recognize multiple human Cytokeratins, including CK10, 13, 14, 15, 16, 18, 19, and it can be used for immunohistochemical detection of tumors from monolayer and multilayered epithelium. |
| Molecular Weight | Observed: 52 kDa,55 kDa |
| UniProt / Swiss-Prot | P13645;P13646;P02533;P19012;P08779;P08727 (Human) |
Recommended dilutions: IHC 1:200-1000;WB 1:500-2000;IF 1:100-500;ELISA 1:1000-5000
Immunogen / Antigen
Synthesized peptide derived from human Cytokeratin Pan AA range:100-200
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse, Rat. Confirm suitability for untested species before use.
Scientific Background
Cytokeratin can label keratinized epithelium, stratified squamous epithelium, stratified epithelium, proliferative keratinized cells and monolayer epithelium. It can be used in the study of various cancers. It is positive in various adenocarcinoma, small cell carcinoma, transitional cell carcinoma, malignant mesothelioma, germ cell tumors other than seminoma, leiomyosarcoma and some synovial sarcomas. CEA and EMA can be combined for the differential diagnosis of epithelial tumors and non epithelial tumors.
Safety & Handling
This antibody is validated for Immunohistochemistry, Western Blot, Immunofluorescence, ELISA. Recommended dilutions: IHC 1:200-1000;WB 1:500-2000;IF 1:100-500;ELISA 1:1000-5000
Confirmed reactivity: Human, Mouse, Rat. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Clone ABT154.
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
