| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | CMD1I;CSM1;CSM2;DES;DESM_HUMAN;Desmin;FLJ12025;FLJ39719;FLJ41013;FLJ41793;Intermediate filament protein;OTTHUMP00000064865 |
| Antigen | |
| Cellular Localization | |
| Clonality | |
| Conjugate | |
| Formulation | |
| Gene ID | |
| Host | |
| Immunogen | Synthesized peptide derived from human Desmin AA range: 400-470 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target |
Product Overview
U-Blot® Desmin (ABT168) Mouse Monoclonal Antibody (WM1414) is a mouse monoclonal antibody from UcallM Biosciences Inc, raised against Desmin. It has been validated for Immunohistochemistry, Western Blot, Immunofluorescence, ELISA and reacts with Human, Mouse, Rat.
Validated Applications
| Target | Desmin |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | ABT168 |
| Isotype | IgG2b, Kappa |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Applications | Immunohistochemistry, Western Blot, Immunofluorescence, ELISA |
| Purification | Protein G |
| Specificity | The antibody can specifically recognize human Desmin protein. |
| Molecular Weight | Calculated: 53 kDa | Observed: 60 kDa |
| UniProt / Swiss-Prot | P17661 (Human) | P31001 (Mouse) | P48675 (Rat) |
Recommended dilutions: IHC 1:200-1000;WB 1:500-2000;IF 1:100-500;ELISA 1:1000-5000
Immunogen / Antigen
Synthesized peptide derived from human Desmin AA range: 400-470
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse, Rat. Confirm suitability for untested species before use.
Scientific Background
Desmin is an intermediate silk protein with a molecular weight of 53 kDa, which is widely distributed in smooth muscle cells, skeletal muscle cells, cardiomyocytes and myofibroblasts. It is mainly used for the diagnosis and differential diagnosis of smooth muscle tumors and striated muscle tumors. In rhabdomyosarcoma, pleomorphic rhabdomyosarcoma was strongly positive, while embryonic / acinar rhabdomyosarcoma was low or even negative. Myofibroblastoma was positive, and the fibroproliferative small round cell tumor showed paranuclear punctate staining.
Safety & Handling
This antibody is validated for Immunohistochemistry, Western Blot, Immunofluorescence, ELISA. Recommended dilutions: IHC 1:200-1000;WB 1:500-2000;IF 1:100-500;ELISA 1:1000-5000
Confirmed reactivity: Human, Mouse, Rat. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Clone ABT168.
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
