| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Cadherin-1;CAM 120/80;Epithelial cadherin;E-cadherin;Uvomorulin;CD324;CDH1;CDHE |
| Antigen | |
| Cellular Localization | |
| Clonality | |
| Conjugate | |
| Formulation | |
| Gene ID | |
| Host | |
| Immunogen | Synthesized peptide derived from human protein. AA range:700-800 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target |
Product Overview
U-Blot® E-cadherin (ABT270) Mouse Monoclonal Antibody (WM1483) is a mouse monoclonal antibody from UcallM Biosciences Inc, raised against E-cadherin. It has been validated for Immunohistochemistry, Western Blot, Immunofluorescence, ELISA and reacts with Human.
Validated Applications
| Target | E-cadherin |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | ABT270 |
| Isotype | IgG2b, Kappa |
| Conjugate | Unconjugated |
| Reactivity | Human |
| Applications | Immunohistochemistry, Western Blot, Immunofluorescence, ELISA |
| Purification | Protein G |
| Specificity | The antibody can specifically recognize E-cadherin protein. |
| Molecular Weight | Calculated: 97 kDa | Observed: 120 kDa |
| UniProt / Swiss-Prot | P12830 (Human) |
Recommended dilutions: IHC 1:200-1000;WB 1:500-2000;IF 1:100-500;ELISA 1:1000-5000
Immunogen / Antigen
Synthesized peptide derived from human protein. AA range:700-800
Reactivity Notes
Confirmed cross-reactivity: Human. Confirm suitability for untested species before use.
Scientific Background
E-Cadherein, 120kDa, is a critical regulator of epithelial junction formation. The E-cadherin internal domain binds with alpha, beta, gamma, and p120 catenins to anchor the ECAD complex to the actin cytoskeleton of the cell. E-cadherin is expressed in virtually all epithelial cells with the exception of adrenocortical cells. The expression in liver cells is weaker than in most other epithelia. ECAD is also expressed in melanocytes (adhering to squamous epithelial cells). Mesothelial cells and granulosa cells are ECAD negative. Mesenchymal cells and neural crest cells are mostly negative but some types may show a weak reaction. Testicular germinative cells are weakly positive. At present, it is commonly used to differentiate breast lobular cancer from ductal cancer (the former is often negative, the latter is often positive).
Safety & Handling
This antibody is validated for Immunohistochemistry, Western Blot, Immunofluorescence, ELISA. Recommended dilutions: IHC 1:200-1000;WB 1:500-2000;IF 1:100-500;ELISA 1:1000-5000
Confirmed reactivity: Human. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Clone ABT270.
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
