| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | CDH1;CDHE;UVO;Cadherin-1;CAM 120/80;Epithelial cadherin;E-cadherin;Uvomorulin;CD antigen CD324 |
| Antigen | |
| Cellular Localization | |
| Clonality | |
| Conjugate | |
| Formulation | |
| Gene ID | |
| Host | |
| Immunogen | Purified recombinant fragment of human E-cadherin expressed in E. Coli. |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target |
Product Overview
U-Blot® E-cadherin Mouse Monoclonal Antibody (WM0150) is a mouse monoclonal antibody from UcallM Biosciences Inc, raised against E-cadherin. It has been validated for Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry, ELISA and reacts with Human, Mouse, Monkey.
Validated Applications
| Target | E-cadherin |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Monkey |
| Applications | Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry, ELISA |
| Purification | Affinity Purified |
| Specificity | E-cadherin Monoclonal Antibody detects endogenous levels of E-cadherin protein. |
| Molecular Weight | Observed: 125-130 kDa |
| UniProt / Swiss-Prot | P12830 (Human) | P09803 (Mouse) |
Recommended dilutions: WB 1:500-1:2000;IHC 1:200-1:1000;Flow Cyt 1:200-1:400;ELISA 1:10000;IF 1:50-200
Immunogen / Antigen
Purified recombinant fragment of human E-cadherin expressed in E. Coli.
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse, Monkey. Confirm suitability for untested species before use.
Scientific Background
This gene encodes a classical cadherin of the cadherin superfamily. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature glycoprotein. This calcium-dependent cell-cell adhesion protein is comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function of this gene is thought to contribute to cancer progression by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. This gene is present in a gene cluster with other members of the cadherin family on chromosome 16. [provided by RefSeq, Nov 2015]
Safety & Handling
This antibody is validated for Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry, ELISA. Recommended dilutions: WB 1:500-1:2000;IHC 1:200-1:1000;Flow Cyt 1:200-1:400;ELISA 1:10000;IF 1:50-200
Confirmed reactivity: Human, Mouse, Monkey. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
