| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Avian erythroblastic leukemia viral;v erb b;oncogene homolog;Cell growth inhibiting protein 40;Cell proliferation inducing protein 61;EGF R;EGFR;EGFR_HUMAN;Epidermal growth factor receptor;avian erythroblastic leukemia viral;v erb b;oncogene homolog;Epidermal growth factor receptor;erythroblastic leukemia viral;v erb b;oncogene homolog avian;Epidermal growth factor receptor;erbb 1;Erbb;Erbb1;ERBB1;Errp;HER1;mENA;Oncogene ERBB;PIG61;Proto-oncogene c-ErbB-1;Receptor tyrosine protein kinase ErbB 1;Receptor tyrosine-protein kinase ErbB-1;Urogastrone;wa2;Wa5. |
| Cellular Localization | |
| Clonality | |
| Conjugate | |
| Formulation | |
| Gene ID | |
| Host | |
| Immunogen | Purified recombinant human EGFR protein fragments expressed in E.coli. |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target |
Product Overview
U-Blot® EGF Receptor Mouse Monoclonal Antibody is a mouse monoclonal antibody from UcallM Biosciences Inc, raised against EGFR. It has been validated for Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation and reacts with Human, Monkey.
Validated Applications
| Target | EGFR |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Conjugate | Unconjugated |
| Reactivity | Human, Monkey |
| Applications | Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation |
| Purification | Affinity Purified |
| Specificity | The antibody detects endogenous level of total EGFR and does not cross-react with related proteins. |
| Molecular Weight | Observed: 175 kDa |
| UniProt / Swiss-Prot | P00533 (Human) | Q01279 (Mouse) |
Recommended dilutions: WB 1:500-1:2000;IHC 1:100-1:300;IF 1:200-1:1000;ELISA 1:5000;IP 1:50-100
Immunogen / Antigen
Purified recombinant human EGFR protein fragments expressed in E.coli.
Reactivity Notes
Confirmed cross-reactivity: Human, Monkey. Confirm suitability for untested species before use.
Scientific Background
The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq, Jun 2016]
Safety & Handling
This antibody is validated for Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation. Recommended dilutions: WB 1:500-1:2000;IHC 1:100-1:300;IF 1:200-1:1000;ELISA 1:5000;IP 1:50-100
Confirmed reactivity: Human, Monkey. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
