U-Blot® Endoplasmin Rabbit Polyclonal Antibody

SKU:BHA21806743
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UcallM Biosciences Inc
UcallM Biosciences Inc
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Overview
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Anti-Endoplasmin antibody (Rabbit host, polyclonal, Unconjugated). Validated for Western Blot, Immunohistochemistry, Immunofluorescence, ELISA with reactivity to Human, Mouse.
Clonality Polyclonal
Host Rabbit
Reactivity Human, Mouse
Conjugate Unconjugated
Isotype IgG
Applications Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
Options selector
Catalog no. Size
WP12186-50 50 uL
WP12186-100 100 uL
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options: Size: 50 uL
  • Lead time: options listed in "Availability Content"; other statuses may take longer.
  • Storage: -15°C to -25°C/1 year(Do not lower than -25°C)
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at recommended temperature as soon as possible.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Mfr No WP12186
Alternative Names HSP90B1;GRP94;TRA1;Endoplasmin;94 kDa glucose-regulated protein;GRP-94;Heat shock protein 90 kDa beta member 1;Tumor rejection antigen 1;gp96 homolog
Antigen
  • Endoplasmin
Cellular Localization Endoplasmic reticulum lumen . Sarcoplasmic reticulum lumen . Melanosome . Identified by mass spectrometry in melanosome fractions from stage I to stage IV. .
Clonality
  • Polyclonal
Concentration 1 mg/ml
Conjugate
  • Unconjugated
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Gene ID 7184 (Human) | 22027 (Mouse)
Host Rabbit
Immunogen The antiserum was produced against synthesized peptide derived from human GRP94. AA range:754-803
Isotype
  • IgG
Molecular Weight Observed: 92 kDa
Product Type
  • Polyclonal Antibody
  • Primary Antibodies
Reactivity
  • Human
  • Mouse
Storage -15°C to -25°C/1 year(Do not lower than -25°C)
Target Endoplasmin
Antibody

Product Overview

U-Blot® Endoplasmin Rabbit Polyclonal Antibody is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against Endoplasmin. It has been validated for Western Blot, Immunohistochemistry, Immunofluorescence, ELISA and reacts with Human, Mouse.

Validated Applications

Target Endoplasmin
Host Rabbit
Clonality Polyclonal
Isotype IgG
Conjugate Unconjugated
Reactivity Human, Mouse
Applications Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
Purification Affinity Purified
Specificity Endoplasmin Polyclonal Antibody detects endogenous levels of Endoplasmin protein.
Molecular Weight Observed: 92 kDa
UniProt / Swiss-Prot P14625 (Human) | P08113 (Mouse)

Recommended dilutions: WB 1:500-1:2000;IHC 1:100-1:300;IF 1:200-1:1000;ELISA 1:20000;Not yet tested in other applications.

Immunogen / Antigen

The antiserum was produced against synthesized peptide derived from human GRP94. AA range:754-803

Reactivity Notes

Confirmed cross-reactivity: Human, Mouse. Confirm suitability for untested species before use.

Scientific Background

This gene encodes a member of a family of adenosine triphosphate(ATP)-metabolizing molecular chaperones with roles in stabilizing and folding other proteins. The encoded protein is localized to melanosomes and the endoplasmic reticulum. Expression of this protein is associated with a variety of pathogenic states, including tumor formation. There is a microRNA gene located within the 5' exon of this gene. There are pseudogenes for this gene on chromosomes 1 and 15. [provided by RefSeq, Aug 2012]

Safety & Handling

For Research Use Only (RUO). Not for diagnostic or therapeutic use. Store as indicated: -15°C to -25°C/1 year(Do not lower than -25°C)
What applications is this antibody validated for?

This antibody is validated for Western Blot, Immunohistochemistry, Immunofluorescence, ELISA. Recommended dilutions: WB 1:500-1:2000;IHC 1:100-1:300;IF 1:200-1:1000;ELISA 1:20000;Not yet tested in other applications.

What species does it react with?

Confirmed reactivity: Human, Mouse. Other species should be validated by the user.

Is this antibody conjugated?

Conjugate: Unconjugated.

How should it be stored?

Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)

Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.

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