| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | EPCAM;GA733-2;M1S2;M4S1;MIC18;TACSTD1;TROP1;Epithelial cell adhesion molecule;Ep-CAM;Adenocarcinoma-associated antigen;Cell surface glycoprotein Trop-1;Epithelial cell surface antigen;Epithelial glycoprotein;EGP;Epithelial gly |
| Antigen | |
| Cellular Localization | |
| Clonality | |
| Conjugate | |
| Formulation | |
| Gene ID | |
| Host | |
| Immunogen | Purified recombinant fragment of human EP-CAM expressed in E. Coli. |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target |
Product Overview
U-Blot® EP-CAM Mouse Monoclonal Antibody (WM0157) is a mouse monoclonal antibody from UcallM Biosciences Inc, raised against Ep-CAM. It has been validated for Western Blot, Immunohistochemistry, Immunofluorescence, ELISA and reacts with Human.
Validated Applications
| Target | Ep-CAM |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Conjugate | Unconjugated |
| Reactivity | Human |
| Applications | Western Blot, Immunohistochemistry, Immunofluorescence, ELISA |
| Purification | Affinity Purified |
| Specificity | EP-CAM Monoclonal Antibody detects endogenous levels of EP-CAM protein. |
| Molecular Weight | Calculated: 35 kDa |
| UniProt / Swiss-Prot | P16422 (Human) | Q99JW5 (Mouse) |
Recommended dilutions: WB 1:500-1:2000;IHC 1:200-1:1000;ELISA 1:10000;IF 1:50-200
Immunogen / Antigen
Purified recombinant fragment of human EP-CAM expressed in E. Coli.
Reactivity Notes
Confirmed cross-reactivity: Human. Confirm suitability for untested species before use.
Scientific Background
This gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy. [provided by RefSeq, Dec 2008]
Safety & Handling
This antibody is validated for Western Blot, Immunohistochemistry, Immunofluorescence, ELISA. Recommended dilutions: WB 1:500-1:2000;IHC 1:200-1:1000;ELISA 1:10000;IF 1:50-200
Confirmed reactivity: Human. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
