| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | FAS;APT1;FAS1;TNFRSF6;Tumor necrosis factor receptor superfamily member 6;Apo-1 antigen;Apoptosis-mediating surface antigen FAS;FASLG receptor;CD antigen CD95 |
| Antigen | |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Conjugate | |
| Formulation | |
| Gene ID | |
| Host | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human FAS. AA range:257-306 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target |
Product Overview
U-Blot® FAS Rabbit Polyclonal Antibody (WP12276) is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against FAS. It has been validated for Western Blot, Immunohistochemistry, Immunofluorescence, ELISA and reacts with Human, Rat, Mouse, Dog.
Validated Applications
| Target | FAS |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human, Rat, Mouse, Dog |
| Applications | Western Blot, Immunohistochemistry, Immunofluorescence, ELISA |
| Purification | Affinity Purified |
| Specificity | FAS Polyclonal Antibody detects endogenous levels of FAS protein. |
| Molecular Weight | Observed: 42 kDa |
| UniProt / Swiss-Prot | P25445 (Human) | P25446 (Mouse) |
Recommended dilutions: WB 1:500-2000;IF 1:50-300;IHC 1:50-300
Immunogen / Antigen
The antiserum was produced against synthesized peptide derived from human FAS. AA range:257-306
Reactivity Notes
Confirmed cross-reactivity: Human, Rat, Mouse, Dog. Confirm suitability for untested species before use.
Scientific Background
The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor contains a death domain. It has been shown to play a central role in the physiological regulation of programmed cell death, and has been implicated in the pathogenesis of various malignancies and diseases of the immune system. The interaction of this receptor with its ligand allows the formation of a death-inducing signaling complex that includes Fas-associated death domain protein (FADD), caspase 8, and caspase 10. The autoproteolytic processing of the caspases in the complex triggers a downstream caspase cascade, and leads to apoptosis. This receptor has been also shown to activate NF-kappaB, MAPK3/ERK1, and MAPK8/JNK, and is found to be involved in transducing the proliferating signals in normal diploid fibroblast and T cells. Several alternatively spliced transcript variants have been described, s
Safety & Handling
This antibody is validated for Western Blot, Immunohistochemistry, Immunofluorescence, ELISA. Recommended dilutions: WB 1:500-2000;IF 1:50-300;IHC 1:50-300
Confirmed reactivity: Human, Rat, Mouse, Dog. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
