| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | 55 kDa actin bundling protein;55 kDa actin-bundling protein;Actin bundling protein;actin bundling protein, 55-KD;FAN 1;FAN1;Fascin 1;Fascin actin bundling protein 1;Fascin;Fascin homolog 1 actin bundling protein;Strongylocentrotus purpuRatus;Fascin homolog 1;Fascin, sea urchin, homolog of, 1;Fascin1;FLJ38511;FSCN 1;FSCN1;FSCN1_HUMAN;HSN;p55;Singed;Drosophila;like;sea urchin fascin homolog like;Singed drosophila homolog like;Singed like;fascin homolog sea urchin;Drosophila;Singed like;fascin homolog sea urchin;Singed like protein;Singed, drosophila, homolog of;Singed-like protein;SNL;Strongylocentrotus purpuRatus |
| Antigen | |
| Cellular Localization | |
| Clonality | |
| Conjugate | |
| Formulation | |
| Gene ID | |
| Host | |
| Immunogen | Synthesized peptide derived from human Fascin AA range: 250-350 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target |
Product Overview
U-Blot® Fascin (ABT-FASN) Mouse Monoclonal Antibody (WM1421) is a mouse monoclonal antibody from UcallM Biosciences Inc, raised against Fascin. It has been validated for Immunohistochemistry, Western Blot, Immunofluorescence, ELISA and reacts with Human, Mouse, Rat.
Validated Applications
| Target | Fascin |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | ABT-FASN |
| Isotype | IgG1, Kappa |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Applications | Immunohistochemistry, Western Blot, Immunofluorescence, ELISA |
| Purification | Protein G |
| Specificity | The antibody can specifically recognize human Fascin protein. In western blotting of Hela cell lysate, the antibody can label a 55 kDa band corresponding to Fascin, while there is no band detected in Fascin knock out Hela cell lysate. |
| Molecular Weight | Calculated: 55 kDa | Observed: 55 kDa |
| UniProt / Swiss-Prot | Q16658 (Human) |
Recommended dilutions: IHC 1:200-1000;WB 1:500-2000;IF 1:100-500;ELISA 1:1000-5000
Immunogen / Antigen
Synthesized peptide derived from human Fascin AA range: 250-350
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse, Rat. Confirm suitability for untested species before use.
Scientific Background
Fascin is a highly conserved actin bundle protein, which is located in microfilaments and stress fibers involved in microfilament bundle formation. It is mainly expressed in dendritic cells and is a sensitive marker of R-S cells. Lymphocytes, plasma cells and myeloid cells did not express. It can be used to diagnose Hodgkin's lymphoma and Langerhans cell histiocytosis.
Safety & Handling
This antibody is validated for Immunohistochemistry, Western Blot, Immunofluorescence, ELISA. Recommended dilutions: IHC 1:200-1000;WB 1:500-2000;IF 1:100-500;ELISA 1:1000-5000
Confirmed reactivity: Human, Mouse, Rat. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Clone ABT-FASN.
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
