| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | FHIT;Bis;5'-adenosyl;-triphosphatase;AP3A hydrolase;AP3Aase;Diadenosine 5';5'''-P1,P3-triphosphate hydrolase;Dinucleosidetriphosphatase;Fragile histidine triad protein |
| Antigen | |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Conjugate | |
| Formulation | |
| Gene ID | |
| Host | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human FHIT. AA range:81-130 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target |
Product Overview
U-Blot® Fhit Rabbit Polyclonal Antibody is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against Fhit. It has been validated for Western Blot, Immunohistochemistry, Immunofluorescence, ELISA and reacts with Human, Rat, Mouse.
Validated Applications
| Target | Fhit |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human, Rat, Mouse |
| Applications | Western Blot, Immunohistochemistry, Immunofluorescence, ELISA |
| Purification | Affinity Purified |
| Specificity | Fhit Polyclonal Antibody detects endogenous levels of Fhit protein. |
| Molecular Weight | Observed: 16 kDa |
| UniProt / Swiss-Prot | P49789 (Human) | O89106 (Mouse) |
Recommended dilutions: WB 1:500-1:2000;IHC 1:100-1:300;IF 1:200-1:1000;ELISA 1:10000;Not yet tested in other applications.
Immunogen / Antigen
The antiserum was produced against synthesized peptide derived from human FHIT. AA range:81-130
Reactivity Notes
Confirmed cross-reactivity: Human, Rat, Mouse. Confirm suitability for untested species before use.
Scientific Background
This gene, a member of the histidine triad gene family, encodes a diadenosine 5',5'''-P1,P3-triphosphate hydrolase involved in purine metabolism. The gene encompasses the common fragile site FRA3B on chromosome 3, where carcinogen-induced damage can lead to translocations and aberrant transcripts of this gene. In fact, aberrant transcripts from this gene have been found in about half of all esophageal, stomach, and colon carcinomas. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Oct 2009]
Safety & Handling
This antibody is validated for Western Blot, Immunohistochemistry, Immunofluorescence, ELISA. Recommended dilutions: WB 1:500-1:2000;IHC 1:100-1:300;IF 1:200-1:1000;ELISA 1:10000;Not yet tested in other applications.
Confirmed reactivity: Human, Rat, Mouse. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
