| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | SH3PXD2A;FISH;KIAA0418;SH3MD1;TKS5;SH3 and PX domain-containing protein 2A;Adapter protein TKS5;Five SH3 domain-containing protein;SH3 multiple domains protein 1;Tyrosine kinase substrate with five SH3 domains |
| Antigen | |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Conjugate | |
| Formulation | |
| Gene ID | |
| Host | |
| Immunogen | Synthesized peptide derived from the N-terminal region of human Fish. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target |
Product Overview
U-Blot® Fish Rabbit Polyclonal Antibody is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against Fish. It has been validated for Immunohistochemistry, Immunofluorescence, Western Blot, ELISA and reacts with Human, Mouse.
Validated Applications
| Target | Fish |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse |
| Applications | Immunohistochemistry, Immunofluorescence, Western Blot, ELISA |
| Purification | Affinity Purified |
| Specificity | Fish Polyclonal Antibody detects endogenous levels of Fish protein. |
| Molecular Weight | Calculated: 125 kDa |
| UniProt / Swiss-Prot | Q5TCZ1 (Human) | O89032 (Mouse) |
Recommended dilutions: IHC 1:100-500;IF/ICC1:100-500;WB 1:500-2000;ELISA 1:5000-20000
Immunogen / Antigen
Synthesized peptide derived from the N-terminal region of human Fish.
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse. Confirm suitability for untested species before use.
Scientific Background
domain:The fifth SH3 domain mediates binding with ADAM12, ADAM15 and ADAM19.,domain:The PX domain is required for podosome localization, and for binding phosphatidylinositol 3-phosphate (PtdIns(3)P) and phosphatidylinositol 3,4-biphosphate (PtdIns(3,4)P2).,function:Required for podosome formation, degradation of the extracellular matrix, and for the invasiveness of some cancer cells. Binds phosphatidylinositol 3-phosphate (PtdIns(3)P) and phosphatidylinositol 3,4-biphosphate (PtdIns(3,4)P2). In association with ADAM12, mediates the neurotoxic effect of beta-amyloid peptide.,PTM:Tyrosine phosphorylated by SRC. Phosphorylation plays a regulatory role in the protein localization. The intramolecular interaction of the PX domain with the third SH3 domain maintains the protein in the cytoplasm and phosphorylation disrupts this interaction, resulting in the redistribution of the protein from cytoplasm to the perimembrane region. Phosphorylated on serine upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the SH3PXD2 family.,similarity:Contains 1 PX (phox homology) domain.,similarity:Contains 5 SH3 domains.,subcellular location:Cytoplasmic in normal cells and localizes to podosomes in SRC-transformed cells.,subunit:Interacts with ADAM12, ADAM15 and ADAM19.,tissue specificity:Found in several cancer cell lines, particularly invasive breast carcinomas and melanomas.
Safety & Handling
This antibody is validated for Immunohistochemistry, Immunofluorescence, Western Blot, ELISA. Recommended dilutions: IHC 1:100-500;IF/ICC1:100-500;WB 1:500-2000;ELISA 1:5000-20000
Confirmed reactivity: Human, Mouse. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
