| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | 90K;90K MAC 2 BP;Basement membrane autoantigen p105;BTBD17B;Galectin 3 binding protein;Galectin-3-binding protein;L3 antigen;Lectin galactoside binding soluble 3 binding protein;Lectin galactoside-binding soluble 3-binding protein;LG3BP_HUMAN;LGALS3BP;M2BP;Mac 2 binding protein;Mac 2 BP;Mac-2 BP;Mac-2-binding protein;Mac2 BP;MAC2BP;Serum protein 90K;TANGO10B;Transport and golgi organization 10 homolog B;Tumor associated antigen 90K;Tumor-associated antigen 90K |
| Antigen | |
| Cellular Localization | |
| Clonality | |
| Conjugate | |
| Formulation | |
| Gene ID | |
| Host | |
| Immunogen | Synthesized peptide derived from human Galectin 3 AA range: 150-250 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target |
Product Overview
U-Blot® Galectin 3 (ABT-GAL3) Mouse Monoclonal Antibody (WM1422) is a mouse monoclonal antibody from UcallM Biosciences Inc, raised against Galectin-3. It has been validated for Immunohistochemistry, Western Blot, Immunofluorescence, ELISA and reacts with Human.
Validated Applications
| Target | Galectin-3 |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | ABT-GAL3 |
| Isotype | IgG2b, Kappa |
| Conjugate | Unconjugated |
| Reactivity | Human |
| Applications | Immunohistochemistry, Western Blot, Immunofluorescence, ELISA |
| Purification | Protein G |
| Specificity | The antibody can specifically recognize human Galectin 3 protein. In western blotting of Hela, A431 and MCF7 cell lysates, the antibody can label a 30 kDa band corresponding to Galectin 3, while there is no band detected in Galectin 3 knock out Hela cell lysate. |
| Molecular Weight | Calculated: 26 kDa | Observed: 33 kDa |
| UniProt / Swiss-Prot | P17931 (Human) |
Recommended dilutions: IHC 1:200-1000;WB 1:500-2000;IF 1:100-500;ELISA 1:1000-5000
Immunogen / Antigen
Synthesized peptide derived from human Galectin 3 AA range: 150-250
Reactivity Notes
Confirmed cross-reactivity: Human. Confirm suitability for untested species before use.
Scientific Background
Galectin-3 is a member of the β-galactose binding lectin family with a molecular weight of 30 kDa which is a chimeric structure consisting of a β-galactoside binding protein and a laminin. The main role of Galectin-3 on the cell surface is related to the adhesion between similar cells. It is expressed in a variety of organs and tissues, including neutrophils and vascular endothelial cells, but it is obviously absent in normal hepatocytes. Galectin-3 can downregulate the expression of some cancers and breast cancer associated proteins, and promote tumor metastasis. It can be used as a reference index for the degree of tumor deterioration.
Safety & Handling
This antibody is validated for Immunohistochemistry, Western Blot, Immunofluorescence, ELISA. Recommended dilutions: IHC 1:200-1000;WB 1:500-2000;IF 1:100-500;ELISA 1:1000-5000
Confirmed reactivity: Human. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Clone ABT-GAL3.
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
