| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | KLF4;EZF;GKLF;Krueppel-like factor 4;Epithelial zinc finger protein EZF;Gut-enriched krueppel-like factor |
| Antigen | |
| Cellular Localization | |
| Clonality | |
| Conjugate | |
| Formulation | |
| Gene ID | |
| Host | |
| Immunogen | Purified recombinant fragment of human GKLF expressed in E. Coli. |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target |
Product Overview
U-Blot® GKLF Mouse Monoclonal Antibody (WM0218) is a mouse monoclonal antibody from UcallM Biosciences Inc, raised against GKLF. It has been validated for Western Blot, Immunohistochemistry, Immunofluorescence, ELISA and reacts with Human.
Validated Applications
| Target | GKLF |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Conjugate | Unconjugated |
| Reactivity | Human |
| Applications | Western Blot, Immunohistochemistry, Immunofluorescence, ELISA |
| Purification | Affinity Purified |
| Specificity | GKLF Monoclonal Antibody detects endogenous levels of GKLF protein. |
| Molecular Weight | Calculated: 55 kDa |
| UniProt / Swiss-Prot | O43474 (Human) | Q60793 (Mouse) |
Recommended dilutions: WB 1:500-1:2000;IHC 1:200-1:1000;IF 1:200-1:1000;ELISA 1:10000;Not yet tested in other applications.
Immunogen / Antigen
Purified recombinant fragment of human GKLF expressed in E. Coli.
Reactivity Notes
Confirmed cross-reactivity: Human. Confirm suitability for untested species before use.
Scientific Background
This gene encodes a protein that belongs to the Kruppel family of transcription factors. The encoded zinc finger protein is required for normal development of the barrier function of skin. The encoded protein is thought to control the G1-to-S transition of the cell cycle following DNA damage by mediating the tumor suppressor gene p53. Mice lacking this gene have a normal appearance but lose weight rapidly, and die shortly after birth due to fluid evaporation resulting from compromised epidermal barrier function. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2015]
Safety & Handling
This antibody is validated for Western Blot, Immunohistochemistry, Immunofluorescence, ELISA. Recommended dilutions: WB 1:500-1:2000;IHC 1:200-1:1000;IF 1:200-1:1000;ELISA 1:10000;Not yet tested in other applications.
Confirmed reactivity: Human. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
