| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | GLUL;GLNS;Glutamine synthetase;GS;Glutamate decarboxylase;Glutamate--ammonia ligase |
| Antigen | |
| Cellular Localization | |
| Clonality | |
| Conjugate | |
| Formulation | |
| Gene ID | |
| Host | |
| Immunogen | AA range: 300-400 |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target |
Product Overview
U-Blot® Gl Syn/Glutamine Synthetase (PTR1400) Mouse Monoclonal Antibody is a mouse monoclonal antibody from UcallM Biosciences Inc, raised against Glutamine synthetase. It has been validated for Western Blot, Immunofluorescence, ELISA, Immunohistochemistry and reacts with Human, Mouse, Rat.
Validated Applications
| Target | Glutamine synthetase |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | PTR1400 |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Applications | Western Blot, Immunofluorescence, ELISA, Immunohistochemistry |
| Purification | Affinity Purified |
| Specificity | This antibody detects endogenous levels of Gl Syn/Glutamine Synthetase protein. |
| Molecular Weight | Calculated: 42 kDa | Observed: 42 kDa |
| UniProt / Swiss-Prot | P15104 (Human) | P15105 (Mouse) | P09606 (Rat) |
Recommended dilutions: IHC 1:200-1000;WB 1:500-2000;IF 1:100-500;ELISA 1:1000-5000
Immunogen / Antigen
AA range: 300-400
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse, Rat. Confirm suitability for untested species before use.
Scientific Background
The protein encoded by this gene belongs to the glutamine synthetase family. It catalyzes the synthesis of glutamine from glutamate and ammonia in an ATP-dependent reaction. This protein plays a role in ammonia and glutamate detoxification, acid-base homeostasis, cell signaling, and cell proliferation. Glutamine is an abundant amino acid, and is important to the biosynthesis of several amino acids, pyrimidines, and purines. Mutations in this gene are associated with congenital glutamine deficiency, and overexpression of this gene was observed in some primary liver cancer samples. There are six pseudogenes of this gene found on chromosomes 2, 5, 9, 11, and 12. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]
Safety & Handling
This antibody is validated for Western Blot, Immunofluorescence, ELISA, Immunohistochemistry. Recommended dilutions: IHC 1:200-1000;WB 1:500-2000;IF 1:100-500;ELISA 1:1000-5000
Confirmed reactivity: Human, Mouse, Rat. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Clone PTR1400.
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
