| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | DGSX;Glypican proteoglycan 3;Glypican-3 [Precursor];Gpc3;GPC3_HUMAN;GTR2 2;GTR2-2;Heparan sulphate proteoglycan;Intestinal protein OCI 5;Intestinal protein OCI-5;MXR7;OCI 5;OCI-5;OCI5;SDYS;Secreted glypican-3;SGB;SGBS;SGBS1 |
| Antigen | |
| Cellular Localization | |
| Clonality | |
| Conjugate | |
| Formulation | |
| Gene ID | |
| Host | |
| Immunogen | Synthesized peptide derived from human Glypican-3(GPC3) AA range: 400-500 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target |
Product Overview
U-Blot® Glypican-3 (ABT180) Mouse Monoclonal Antibody (WM1426) is a mouse monoclonal antibody from UcallM Biosciences Inc, raised against Glypican-3. It has been validated for Immunohistochemistry, Western Blot, Immunofluorescence, ELISA and reacts with Human.
Validated Applications
| Target | Glypican-3 |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | ABT180 |
| Isotype | IgG1, Kappa |
| Conjugate | Unconjugated |
| Reactivity | Human |
| Applications | Immunohistochemistry, Western Blot, Immunofluorescence, ELISA |
| Purification | Protein G |
| Specificity | The antibody can specifically recognize human Glypican-3 protein. |
| Molecular Weight | Calculated: 66 kDa | Observed: 58 kDa |
| UniProt / Swiss-Prot | P51654 (Human) |
Recommended dilutions: IHC 1:200-1000;WB 1:500-2000;IF 1:100-500;ELISA 1:1000-5000
Immunogen / Antigen
Synthesized peptide derived from human Glypican-3(GPC3) AA range: 400-500
Reactivity Notes
Confirmed cross-reactivity: Human. Confirm suitability for untested species before use.
Scientific Background
Glypican 3 is a carcinoembryonic protein and belongs to the heparan sulfate polysaccharide family. It is mainly expressed in fetal liver, kidney and trophoblast, but not in other normal tissues. Glypican 3 is a sensitive and specific marker of hepatocellular carcinoma. Its expression is related to differentiation. The lower the degree of differentiation, the higher the expression. It is highly expressed in ovarian yolk sac tumor, choriocarcinoma and melanoma.
Safety & Handling
This antibody is validated for Immunohistochemistry, Western Blot, Immunofluorescence, ELISA. Recommended dilutions: IHC 1:200-1000;WB 1:500-2000;IF 1:100-500;ELISA 1:1000-5000
Confirmed reactivity: Human. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Clone ABT180.
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
