| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | BLZF1;JEM1;Golgin-45;Basic leucine zipper nuclear factor 1;JEM-1;p45 basic leucine-zipper nuclear factor |
| Antigen | |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Conjugate | |
| Formulation | |
| Gene ID | |
| Host | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human BLZF1. AA range:10-59 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target |
Product Overview
U-Blot® Golgin 45 Rabbit Polyclonal Antibody is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against Golgin 45. It has been validated for Western Blot, Immunohistochemistry, Immunofluorescence, ELISA and reacts with Human, Mouse.
Validated Applications
| Target | Golgin 45 |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse |
| Applications | Western Blot, Immunohistochemistry, Immunofluorescence, ELISA |
| Purification | Affinity Purified |
| Specificity | Golgin 45 Polyclonal Antibody detects endogenous levels of Golgin 45 protein. |
| Molecular Weight | Observed: 47 kDa |
| UniProt / Swiss-Prot | Q9H2G9 (Human) | Q8R2X8 (Mouse) |
Recommended dilutions: WB 1:500-1:2000;IHC 1:100-1:300;IF 1:200-1:1000;ELISA 1:20000;Not yet tested in other applications.
Immunogen / Antigen
The antiserum was produced against synthesized peptide derived from human BLZF1. AA range:10-59
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse. Confirm suitability for untested species before use.
Scientific Background
caution:Because of the presence of a potential basic motif and leucine-zipper domain, PubMed:9129147 and PubMed:11056056 have thought that BLZF1 is a potential transcription factor. They found it localized in the nucleus, except isoform 2, which was cytoplasmic. However, homology at several typical position for basic or hydrophobic residues is missing.,function:Required for normal Golgi structure and for protein transport from the endoplasmic reticulum (ER) through the Golgi apparatus to the cell surface.,induction:Up-regulated by retinoids.,subunit:Interacts with GORASP2 and with the GTP-bound form of RAB2, but not with other Golgi Rab proteins. GORASP2 and BLZF1 form a RAB2 effector complex on medial Golgi.,tissue specificity:Ubiquitous. Also found in cell lines derived from several hematopoietic pathologies, such as T-cell leukemia, pro-B, pre-B, myeloma, and plasmacytoma cell lines, but not in Burkitt lymphoma cells.
Safety & Handling
This antibody is validated for Western Blot, Immunohistochemistry, Immunofluorescence, ELISA. Recommended dilutions: WB 1:500-1:2000;IHC 1:100-1:300;IF 1:200-1:1000;ELISA 1:20000;Not yet tested in other applications.
Confirmed reactivity: Human, Mouse. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
