| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | HIF1A;BHLHE78;MOP1;PASD8;Hypoxia-inducible factor 1-alpha;HIF-1-alpha;HIF1-alpha;ARNT-interacting protein;Basic-helix-loop-helix-PAS protein MOP1;Class E basic helix-loop-helix protein 78;bHLHe78;Member of PAS protein 1;PAS doma |
| Antigen | |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Conjugate | |
| Formulation | |
| Gene ID | |
| Host | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human HIF-1alpha. AA range:328-377 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target |
Product Overview
U-Blot® HIF-1α Rabbit Polyclonal Antibody is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against HIF-1α. It has been validated for Immunofluorescence, Western Blot, Immunohistochemistry, Immunoprecipitation, ELISA and reacts with Human, Mouse, Rat.
Validated Applications
| Target | HIF-1α |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Applications | Immunofluorescence, Western Blot, Immunohistochemistry, Immunoprecipitation, ELISA |
| Purification | Affinity Purified |
| Specificity | HIF-1α Polyclonal Antibody detects endogenous levels of HIF-1α protein. |
| Molecular Weight | Observed: 92-130 kDa |
| UniProt / Swiss-Prot | Q16665 (Human) | Q61221 (Mouse) | O35800 (Rat) |
Recommended dilutions: IF 1:50-200;WB 1:500-1:2000;IP 1:200;IHC 1:100-1:300;ELISA 1:40000;Not yet tested in other applications
Immunogen / Antigen
The antiserum was produced against synthesized peptide derived from human HIF-1alpha. AA range:328-377
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse, Rat. Confirm suitability for untested species before use.
Scientific Background
hypoxia inducible factor 1 alpha subunit(HIF1A) Homo sapiens This gene encodes the alpha subunit of transcription factor hypoxia-inducible factor-1 (HIF-1), which is a heterodimer composed of an alpha and a beta subunit. HIF-1 functions as a master regulator of cellular and systemic homeostatic response to hypoxia by activating transcription of many genes, including those involved in energy metabolism, angiogenesis, apoptosis, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. HIF-1 thus plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jul 2011]
Safety & Handling
This antibody is validated for Immunofluorescence, Western Blot, Immunohistochemistry, Immunoprecipitation, ELISA. Recommended dilutions: IF 1:50-200;WB 1:500-1:2000;IP 1:200;IHC 1:100-1:300;ELISA 1:40000;Not yet tested in other applications
Confirmed reactivity: Human, Mouse, Rat. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
