| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | ARNT;BHLHE2;Aryl hydrocarbon receptor nuclear translocator;ARNT protein;Class E basic helix-loop-helix protein 2;bHLHe2;Dioxin receptor, nuclear translocator;Hypoxia-inducible factor 1-beta;HIF-1-beta;HIF1-beta |
| Antigen | |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Conjugate | |
| Formulation | |
| Gene ID | |
| Host | |
| Immunogen | Recombinant Protein of HIF-1 β |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target |
Product Overview
U-Blot® HIF-1 β/ARNT Mouse Monoclonal Antibody (4C5) is a mouse monoclonal antibody from UcallM Biosciences Inc, raised against HIF-1β. It has been validated for Western Blot, Immunohistochemistry, Immunofluorescence and reacts with Human, Mouse, Rat.
Validated Applications
| Target | HIF-1β |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 4C5 |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Applications | Western Blot, Immunohistochemistry, Immunofluorescence |
| Purification | Affinity Purified |
| Specificity | HIF-1 β/ARNT protein detects endogenous levels of HIF-1 β/ARNT |
| Molecular Weight | Observed: 87 kDa |
| UniProt / Swiss-Prot | P27540 (Human) | P53762 (Mouse) |
Recommended dilutions: WB 1:1000-2000;IHC 1:100-200;IF 1:50-200
Immunogen / Antigen
Recombinant Protein of HIF-1 β
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse, Rat. Confirm suitability for untested species before use.
Scientific Background
This gene encodes a protein containing a basic helix-loop-helix domain and two characteristic PAS domains along with a PAC domain. The encoded protein binds to ligand-bound aryl hydrocarbon receptor and aids in the movement of this complex to the nucleus, where it promotes the expression of genes involved in xenobiotic metabolism. This protein is also a co-factor for transcriptional regulation by hypoxia-inducible factor 1. Chromosomal translocation of this locus with the ETV6 (ets variant 6) gene on chromosome 12 have been described in leukemias. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2013]
Safety & Handling
This antibody is validated for Western Blot, Immunohistochemistry, Immunofluorescence. Recommended dilutions: WB 1:1000-2000;IHC 1:100-200;IF 1:50-200
Confirmed reactivity: Human, Mouse, Rat. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Clone 4C5.
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
