| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | NOS2;NOS2A;Nitric oxide synthase;inducible;Hepatocyte NOS;HEP-NOS;Inducible NO synthase;Inducible NOS;iNOS;NOS type II;Peptidyl-cysteine S-nitrosylase NOS2 |
| Antigen | |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Conjugate | |
| Formulation | |
| Gene ID | |
| Host | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human iNOS. AA range:117-166 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target |
Product Overview
U-Blot® iNOS Rabbit Polyclonal Antibody is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against NOS2/iNOS. It has been validated for Immunofluorescence, Western Blot, Immunohistochemistry, ELISA and reacts with Human, Mouse, Rat.
Validated Applications
| Target | NOS2/iNOS |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Applications | Immunofluorescence, Western Blot, Immunohistochemistry, ELISA |
| Purification | Affinity Purified |
| Specificity | NOS2 Polyclonal Antibody detects endogenous levels of NOS2 protein. |
| Molecular Weight | Observed: 131 kDa |
| UniProt / Swiss-Prot | P35228 (Human) | P29477 (Mouse) | Q06518 (Rat) |
Recommended dilutions: IF 1:50-200;WB 1:500-2000;ELISA 1:10000-20000;IHC 1:50-300
Immunogen / Antigen
The antiserum was produced against synthesized peptide derived from human iNOS. AA range:117-166
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse, Rat. Confirm suitability for untested species before use.
Scientific Background
Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. This gene encodes a nitric oxide synthase which is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. Three related pseudogenes are located within the Smith-Magenis syndrome region on chromosome 17. [provided by RefSeq, Jul 2008]
Safety & Handling
This antibody is validated for Immunofluorescence, Western Blot, Immunohistochemistry, ELISA. Recommended dilutions: IF 1:50-200;WB 1:500-2000;ELISA 1:10000-20000;IHC 1:50-300
Confirmed reactivity: Human, Mouse, Rat. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
