| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | JUN;Transcription factor AP-1;Activator protein 1;AP1;Proto-oncogene c-Jun;V-jun avian sarcoma virus 17 oncogene homolog;p39;JUND;Transcription factor jun-D |
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| Cellular Localization | |
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| Conjugate | |
| Formulation | |
| Gene ID | |
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Product Overview
U-Blot® Jun/JunD (Phospho Ser73/Ser100) Rabbit Monoclonal Antibody is a rabbit monoclonal antibody from UcallM Biosciences Inc, raised against c-Jun. Recognizes Phospho (Ser73/100). It has been validated for Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation, ELISA and reacts with Human, Mouse, Rat.
Validated Applications
| Target | c-Jun |
|---|---|
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone | PT0912R |
| Isotype | IgG, Kappa |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Applications | Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation, ELISA |
| Purification | Protein A |
| Specificity | Phospho-Jun/JunD (Ser73/Ser100) Antibody detects endogenous levels of c JUN protein only when phosphorylated at S73/100.The name of modified sites may be influenced by many factors, such as species (the modified site was not originally found in human samples) and the change of protein sequence (the previous protein sequence is incomplete, and the protein sequence may be prolonged with the development of protein sequencing technology). When naming, we will use the "numbers" in historical reference to keep the sites consistent with the reports. The antibody binds to the following modification sequence (lowercase letters are modification sites):CSSVs |
| Molecular Weight | Calculated: 35 kDa,36 kDa | Observed: 42 kDa,48 kDa |
| UniProt / Swiss-Prot | P05412;P17535 (Human) | P17325;P52909 (Rat) |
Recommended dilutions: IHC 1:200-1:1000;WB 1:2000-1:10000;IF 1:200-1:1000;ELISA 1:5000-1:20000;IP 1:50-1:200
Immunogen / Antigen
Transcription factor AP-1;jun;c-jun；AP-1; Transcription factor jun-D
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse, Rat. Confirm suitability for untested species before use.
Scientific Background
This gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies. [provided by RefSeq, Jul 2008]
Safety & Handling
This antibody is validated for Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation, ELISA. Recommended dilutions: IHC 1:200-1:1000;WB 1:2000-1:10000;IF 1:200-1:1000;ELISA 1:5000-1:20000;IP 1:50-1:200
Confirmed reactivity: Human, Mouse, Rat. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Clone PT0912R.
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
