U-Blot® MaxSignal ECL Solution

Product Overview

U-Blot® MaxSignal ECL Solution is a highly sensitive, non-radioactive chemiluminescent substrate based on Luminol for detecting horseradish peroxidase (HRP) immobilized on membranes during immunoblotting, with the ability to detect antigens at femtogram-level antigens.

The continuous luminescence lasts up to 6 hours and can be repeatedly exposed to chemiluminescent imaging systems for optimal results, as well as used for stripping immunodetection reagents and reprobing.

Specifications

Applications

Used to detect HRP-labeled probes or antibodies in Western Blot, Northern Blot, Southern Blot, Dot Blot.

Compatibility & Usage Notes

Mix the two components in equal volumes (1:1) immediately before use and warm to room temperature. Keep the membrane wet, incubate 3-5 minutes, and avoid excess HRP, which can quench signal.

Safety & Handling

Instructions for Use

• Perform routine electrophoresis, transfer, incubation with HRP-labeled antibodies or nucleic-acid probes, and membrane washing.
• During the last wash, freshly prepare working solution by mixing equal volumes (1:1) of Luminol/Enhancer solution and Stable Peroxide Buffer; warm to room temperature and use immediately for best results.
• Drain the membrane on filter paper (do not let it dry) and fully immerse it in working solution (~0.125 ml/cm²). Incubate 3-5 minutes at room temperature, then expose. Do not over-incubate.
• Drain excess solution and place the blot in the imaging device per the instrument instructions, then expose for seconds to minutes.

Technical Notes

• Avoid milk powder in the blocking solution when using avidin/biotin systems (milk contains endogenous biotin).
• Add Tween-20 (final 0.05%) to blocking buffer and antibody diluents to reduce nonspecific binding.
• Do not use sodium azide as a preservative; it inhibits HRP.
• Keep the membrane wet throughout and use a shaker during incubation and washing.
• The prepared working solution is stable at room temperature for up to 8 hours in the dark; use a brown bottle to limit light exposure.

Key Features

Highly sensitive Luminol-based chemiluminescent substrate; up to 6 hours of continuous signal; compatible with repeated exposure, stripping, and reprobing.