| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Beckwith Wiedemann syndrome;BWCR;BWS;CDKI;CDKN 1C;CDKN1C;CDN1C_HUMAN;Cyclin dependent kinase inhibitor 1C;Cyclin dependent kinase inhibitor p57;Cyclin-dependent kinase inhibitor 1C;Cyclin-dependent kinase inhibitor p57;KIP 2;KIP2;p57;p57 Kip 2;p57KIP2;WBS |
| Antigen | |
| Cellular Localization | |
| Clonality | |
| Conjugate | |
| Formulation | |
| Gene ID | |
| Host | |
| Immunogen | Synthesized peptide derived from human protein. AA range:200-316 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target |
Product Overview
U-Blot® p57kip2 (ABT214) Mouse Monoclonal Antibody (WM1441) is a mouse monoclonal antibody from UcallM Biosciences Inc, raised against p57. It has been validated for Immunohistochemistry, Western Blot, Immunofluorescence, ELISA and reacts with Human, Mouse, Rat.
Validated Applications
| Target | p57 |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | ABT214 |
| Isotype | IgG2b, Kappa |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Applications | Immunohistochemistry, Western Blot, Immunofluorescence, ELISA |
| Purification | Protein G |
| Specificity | The antibody can specifically recognize human p57kip2 protein. |
| Molecular Weight | Calculated: 50 kDa | Observed: 57 kDa |
| UniProt / Swiss-Prot | P49918 (Human) | P49919 (Mouse) |
Recommended dilutions: IHC 1:200-1000;WB 1:500-2000;IF 1:100-500;ELISA 1:1000-5000
Immunogen / Antigen
Synthesized peptide derived from human protein. AA range:200-316
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse, Rat. Confirm suitability for untested species before use.
Scientific Background
P57kip2 is a cell cycle inhibitor and tumor suppressor. The gene is mainly located on the mother's chromosome 11. By blocking the G1 / S phase transition in the cell cycle, we can realize the negative regulation of the cell cycle, and then prevent cell proliferation and tumor formation. In complete hydatidiform mole, there is a lack of maternal gene and p57 negative expression, while in partial hydatidiform mole, there are genes of both parents and p57 positive expression, which can be used for the study of early hydatidiform mole pregnancy.
Safety & Handling
This antibody is validated for Immunohistochemistry, Western Blot, Immunofluorescence, ELISA. Recommended dilutions: IHC 1:200-1000;WB 1:500-2000;IF 1:100-500;ELISA 1:1000-5000
Confirmed reactivity: Human, Mouse, Rat. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Clone ABT214.
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
