| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | NEF；Protein S100 B；Protein S100-B；S 100 calcium binding protein beta chain；S 100 protein beta chain；S-100 protein beta chain；S-100 protein subunit beta；S100；S100 calcium binding protein beta (neural)；S100 calcium-binding protein B；S100 protein beta chain；S100B；S100B_HUMAN；S100beta |
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| Clonality | |
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| Gene ID | |
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Product Overview
U-Blot® S100B Rabbit Monoclonal Antibody is a rabbit monoclonal antibody from UcallM Biosciences Inc, raised against S100B. It has been validated for Western Blot, Immunohistochemistry, Immunofluorescence, ELISA and reacts with Human, Mouse, Rat.
Validated Applications
| Target | S100B |
|---|---|
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone | PT0461R |
| Isotype | IgG, Kappa |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Applications | Western Blot, Immunohistochemistry, Immunofluorescence, ELISA |
| Purification | Protein A |
| Specificity | Endogenous |
| Molecular Weight | Calculated: 11 kDa | Observed: 11 kDa |
| UniProt / Swiss-Prot | P04271 (Human) | P50114 (Mouse) |
Recommended dilutions: IHC 1:400-1:2000;WB 1:2000-1:10000;IF 1:200-1:1000;ELISA 1:5000-1:20000
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse, Rat. Confirm suitability for untested species before use.
Scientific Background
The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21; however, this gene is located at 21q22.3. This protein may function in Neurite extension, proliferation of melanoma cells, stimulation of Ca2+ fluxes, inhibition of PKC-mediated phosphorylation, astrocytosis and axonal proliferation, and inhibition of microtubule assembly. Chromosomal rearrangements and altered expression of this gene have been implicated in several neurological, neoplastic, and other types of diseases, including Alzheimer's disease, Down's syndrome, epilepsy
Safety & Handling
This antibody is validated for Western Blot, Immunohistochemistry, Immunofluorescence, ELISA. Recommended dilutions: IHC 1:400-1:2000;WB 1:2000-1:10000;IF 1:200-1:1000;ELISA 1:5000-1:20000
Confirmed reactivity: Human, Mouse, Rat. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Clone PT0461R.
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
