| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | INPP5D;SHIP;SHIP1;Phosphatidylinositol 3;4,5-trisphosphate 5-phosphatase 1;Inositol polyphosphate-5-phosphatase of 145 kDa;SIP-145;SH2 domain-containing inositol 5'-phosphatase 1;SH2 domain-containing inositol phosphatase 1;SHIP-1 |
| Antigen | |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Conjugate | |
| Formulation | |
| Gene ID | |
| Host | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human SHIP1 around the phosphorylation site of Tyr1021. AA range:987-1036 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target |
Product Overview
U-Blot® SHIP-1 (Phospho Tyr1021) Rabbit Polyclonal Antibody is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against SHIP-1. Recognizes Phospho. It has been validated for Western Blot, Immunofluorescence, ELISA, Immunohistochemistry and reacts with Human, Mouse, Rat.
Validated Applications
| Target | SHIP-1 |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Applications | Western Blot, Immunofluorescence, ELISA, Immunohistochemistry |
| Purification | Affinity Purified |
| Specificity | Phospho-SHIP-1 (Y1021) Polyclonal Antibody detects endogenous levels of SHIP-1 protein only when phosphorylated at Y1021. |
| Molecular Weight | Observed: 133 kDa |
| UniProt / Swiss-Prot | Q92835 (Human) | Q9ES52 (Mouse) | P97573 (Rat) |
Recommended dilutions: WB 1:500-2000;IF/ICC1:50-200;ELISA 1:2000-20000;IHC 1:50-200
Immunogen / Antigen
The antiserum was produced against synthesized peptide derived from human SHIP1 around the phosphorylation site of Tyr1021. AA range:987-1036
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse, Rat. Confirm suitability for untested species before use.
Scientific Background
This gene is a member of the inositol polyphosphate-5-phosphatase (INPP5) family and encodes a protein with an N-terminal SH2 domain, an inositol phosphatase domain, and two C-terminal protein interaction domains. Expression of this protein is restricted to hematopoietic cells where its movement from the cytosol to the plasma membrane is mediated by tyrosine phosphorylation. At the plasma membrane, the protein hydrolyzes the 5' phosphate from phosphatidylinositol (3,4,5)-trisphosphate and inositol-1,3,4,5-tetrakisphosphate, thereby affecting multiple signaling pathways. The protein is also partly localized to the nucleus, where it may be involved in nuclear inositol phosphate signaling processes. Overall, the protein functions as a negative regulator of myeloid cell proliferation and survival. Mutations in this gene are associated with defects and cancers of the immune system. A
Safety & Handling
This antibody is validated for Western Blot, Immunofluorescence, ELISA, Immunohistochemistry. Recommended dilutions: WB 1:500-2000;IF/ICC1:50-200;ELISA 1:2000-20000;IHC 1:50-200
Confirmed reactivity: Human, Mouse, Rat. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
