| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human UBR5 recombinant protein (Position: K31-K761) was used as the immunogen for the UBR5 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
UBR5 Antibody / hHYD is a anti-UBR5 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: UBR5
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, FACS, ELISA
Biological background
UBR5 is encoded by the UBR5 gene located on human chromosome 8q22.3. The protein is approximately 300 kilodaltons and contains a C-terminal HECT domain responsible for ubiquitin transfer, an N-terminal UBR box involved in substrate recognition, and several WW domains that mediate protein-protein interactions. UBR5 localizes to both the nucleus and cytoplasm, functioning in proteasomal degradation and signaling complex assembly.
The UBR5 antibody detects a 300 kilodalton band in western blot analysis and shows both nuclear and cytoplasmic staining under immunofluorescence. UBR5 ubiquitinates regulators of the DNA damage response such as BRCA1 and p53, coordinating checkpoint activation and repair. It also targets transcription factors and signaling intermediates, integrating stress responses with transcriptional control.
UBR5 is essential for embryonic development and cell viability. Its dysregulation is associated with multiple cancers, including breast, ovarian, and colorectal carcinoma, where it functions as either an oncogene or tumor suppressor depending on context. Overexpression enhances proliferation and metastasis, while depletion sensitizes cells to DNA-damaging agents and promotes apoptosis.
As a master regulator of ubiquitin signaling, UBR5 links proteolytic control with cell fate decisions.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
