| Field | Specification |
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| Immunogen | A synthesized peptide derived from human UBF1 was used as the immunogen for the UBTF antibody. |
| Isotype | |
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Overview
UBTF Antibody / UBF1 / Upstream binding factor 1 is a anti-UBTF Rabbit antibody Recombinant Rabbit Monoclonal clone 32U20 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC) with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: UBTF
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 32U20, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB, IHC
Biological background
Upstream binding factor 1 binds ribosomal DNA promoter sequences and induces conformational changes that promote recruitment of RNA polymerase I and associated factors. Research using UBTF antibody has shown that the protein cooperates with selectivity factor 1 and other nucleolar proteins to form the transcription initiation complex. By bending ribosomal DNA and stabilizing transcription machinery, Upstream binding factor 1 ensures efficient expression of ribosomal RNA, a prerequisite for ribosome assembly and cellular protein synthesis.
Studies with UBTF antibody have demonstrated that the protein is subject to regulation by post-translational modifications. Phosphorylation by kinases such as ERK and mTOR influences DNA binding activity and transcriptional output. This regulation allows cells to couple ribosome biogenesis with growth signals, ensuring that protein production capacity matches proliferative demands. Such control is critical during development, stress adaptation, and oncogenic transformation.
In addition to transcriptional regulation, Upstream binding factor 1 contributes to nucleolar structure. Research using UBTF antibody has revealed that UBTF localizes to transcriptionally active nucleolar regions, forming foci that align with ribosomal DNA arrays. Loss of UBTF disrupts nucleolar organization, reduces ribosomal RNA transcription, and impairs ribosome assembly. These structural roles highlight UBTF as both a functional transcription factor and a nucleolar architectural protein.
Dysregulation of UBTF has been implicated in cancer and neurodegenerative disease. Studies with UBTF antibody have shown that elevated UBTF activity supports increased ribosome biogenesis in tumors, fueling uncontrolled cell proliferation. Overexpression correlates with poor prognosis in certain cancers, making UBTF a potential diagnostic marker and therapeutic target. Conversely, mutations in UBTF have been associated with pediatric neurodevelopmental syndromes and neurodegeneration, linking impaired ribosome production to neuronal dysfunction. These findings underscore the dual importance of UBTF in health and disease.
Upstream binding factor 1 also interacts with chromatin remodeling factors. Research using UBTF antibody has demonstrated that UBTF recruits or cooperates with histone modifiers to establish transcriptionally competent rDNA chromatin. Through these interactions, UBTF integrates epigenetic cues with transcriptional output, providing a mechanism for long-term regulation of ribosome synthesis in response to environmental signals.
UBTF antibody is widely applied in chromatin immunoprecipitation, immunofluorescence, and western blotting. Chromatin immunoprecipitation identifies UBTF binding across ribosomal DNA repeats, immunofluorescence demonstrates nucleolar localization, and western blotting confirms expression levels and post-translational modifications. These experimental applications make UBTF antibody indispensable for studying transcriptional control of ribosome biogenesis.
By supplying validated UBTF antibody reagents,
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
