| Field | Specification |
|---|---|
| Mfr No | |
| Product Type | |
| Shipping | |
| Source | Recombinant (E. coli) |
| Storage |
UCF.METM Thermostable RNase H, a thermostable ribonuclease endonuclease, exhibits specific activity at elevated temperatures, targeting the phosphodiester bonds within RNA sequences of RNA:DNA hybrid structures while preserving the integrity of DNA sequences. Furthermore, it does not degrade single-stranded or double-stranded RNA or DNA substrates. Despite sharing similar ribonuclease endonuclease characteristics with its E. coli counterpart, the thermostable enzyme demonstrates enhanced activity at higher temperatures, with optimal functionality exceeding 65oC. This attribute enables UCF.METM Thermostable RNase H to be utilized in high-temperature experimental settings, ensuring heightened sensitivity and minimizing nonspecific hybridization backgrounds.
Yeasen offers comprehensive solutions for experiments. Explore our related products: Ultra-Clean UCF.ME® Thermostable RNase H: Precise Cutting for More Efficient Experiments!
Features
High activity and excellent batch-to-batch consistency.
Low host gDNA residue: <0.02 copies/U.
Free of exonuclease, nickase, and RNase residue.
Superior stability: No significant decrease in enzyme activity after 32 days at 4oC, 16 days at 25oC, or 7 days at 37oC.
Applications
rRNA removal.
Detection of mRNA capping efficiency.
Removal of mRNA poly (A) hybridized to poly (dT).
Elimination of mRNA during cDNA second-strand synthesis.
Enhancement of amplification efficiency in isothermal amplification experiments.
Specifications
|
Cat.No. |
14545ES72 / 14545ES82 |
|
Size |
250 U / 1250 U |
|
Concentration |
5 U/μL |
|
Unit Definition |
One unit is defined as the amount of enzyme required to produce 1 nmol of ribonucleotides from 40 picomoles of a fluorescently labeled 25 base pair RNA-DNA hybrid in a total reaction volume of 50 µl in 20 minutes at 50oC |
|
Reaction conditions |
≥50oC |
Glycerol Content |
Contains Glycerol |
Components
|
Components No. |
Name |
14545ES72 |
14545ES82 |
|
14545-A |
UCF.METM Thermostable RNase H (5 U/μL) |
50 μL |
250 μL |
|
14545-B |
10×RNase H Reaction Buffer |
1 mL |
5×1 mL |
Shipping and Storage
This product should be stored at -25 ~ -15oC for 1 year.
Figures
Figure 1. Activity Detection Results of UCF.METM Thermostable RNase H.
Note: Reaction conditions: 50°C for 20 minutes; RNA:DNA substrate - 20 pmol.
Figure 2. Host gDNA Residue Results of UCF.METM Thermostable RNase H.
Figure 3. Free of exonuclease, nickase, and RNase residue.
Figure 4. Accelerated Stability Results of UCF.METM Thermostable RNase H.
Store this enzyme at -20°C and avoid repeated freeze-thaw cycles to preserve catalytic activity. The product is shipped Dry Ice and remains stable for up to one year from the date of manufacture when stored under recommended conditions. Aliquoting the stock solution into single-use volumes is recommended for enzymes used infrequently to minimize thermal cycling of the bulk stock.
rRNA removal . Detection of mRNA capping efficiency . Removal of mRNA poly (A) hybridized to poly (dT) . Elimination of mRNA during cDNA second-strand synthesis. Enhancement of amplification efficiency in isothermal amplification experiments .. Always verify compatibility with your specific template, buffer, and downstream workflow.
One unit (U) is defined as the amount of enzyme that degrades 1 µg of DNA or RNA substrate to acid-soluble form in 30 min at 37°C under defined buffer conditions.
This enzyme is produced as Recombinant (E. coli) and supplied as a Research Use Only (RUO) reagent. Each lot is subjected to activity assay, purity assessment by SDS-PAGE, and functional validation prior to release. A Certificate of Analysis (CoA) and Safety Data Sheet (SDS) are available on request.
Nuclease activity typically requires divalent metal ion cofactors (Mg²⁺ or Mn²⁺ at 1–5 mM). Activity is inhibited by chelating agents such as EDTA (≥1 mM), high salt concentrations, and reducing agents such as DTT at elevated concentrations. Heat inactivation (65–75°C for 15 min) is effective for most nucleases, allowing removal of enzyme activity after digestion without column purification.
Yeasen Biotechnology supports custom enzyme solutions across multiple service lines — from GMP-grade bulk supply to directed enzyme engineering. Contact BioHippo to discuss requirements and initiate a project inquiry.
▶ GMP-Grade & Bulk Supply
Select Yeasen enzymes are available in GMP grade, manufactured in an ISO 13485-certified UCF.ME™ ultra-clean molecular enzyme facility with FDA Drug Master File (DMF) support.
- GMP-grade release testing and CoA documentation
- ISO 13485-certified production facility
- Scalable from milligram to multi-gram quantities
- Consistent lot-to-lot activity specifications
▶ Glycerol-Free & Custom Formulation
Glycerol-free enzyme formats are available for applications requiring lyophilization compatibility, liquid handling automation, or direct IVD master mix integration.
- Glycerol-free liquid format (standard and custom buffers)
- Lyophilization-ready enzyme preparation
- Custom reaction buffer optimization for specific assay conditions
- Compatible with freeze-drying workflows for point-of-care formats
▶ Molecular IVD RDC Service
Yeasen's Research and Development Contracting (RDC) team delivers end-to-end solutions for molecular diagnostic product development, covering enzyme selection through clinical validation support.
- Enzyme selection and performance matching
- Primer/probe design and reaction buffer optimization
- Sensitivity, specificity, and precision validation studies
- Stability studies and SNP evaluation
- Instrument platform compatibility assessment
▶ ZymeEditor™ Enzyme Engineering
Yeasen's proprietary ZymeEditor™ directed evolution and rational design platform enables the development of custom enzyme variants with tailored performance characteristics not available in off-the-shelf products.
- Directed evolution for enhanced thermostability, processivity, or fidelity
- Rational design for altered substrate specificity or cofactor requirements
- Library screening from Yeasen's proprietary enzyme variant collection
- Scale-up to commercial quantities upon candidate confirmation
ⓘ Customization services are fulfilled by Yeasen Biotechnology. Lead times and minimum order quantities vary by service type. Contact BioHippo for project scoping and pricing.
