| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A peptide specific to Up-frameshift suppressor 1 protein was used as the immunogen for the UPF1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
UPF1 Antibody / Up-frameshift suppressor 1 / RENT1 is a anti-UPF1 Mouse antibody Recombinant Rabbit Monoclonal clone 19U80 supplied in Antigen affinity purified format. Recommended for workflows such as Western blot (WB), IHC-P with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasmic, Nuclear.
Key elements and design rationale
- Target: UPF1
- Antibody details: Mouse, Recombinant Rabbit Monoclonal, clone 19U80, isotype Rabbit IgG
- Format: Antigen affinity purified
- Applications (as listed): WB, IHC-P
Biological background
UPF1 associates with the exon junction complex and interacts with other NMD factors, including UPF2 and UPF3, to form surveillance complexes on target transcripts. Its helicase activity enables it to remodel RNA protein complexes, while its phosphorylation state determines whether bound transcripts are degraded or stabilized. Beyond NMD, UPF1 has also been implicated in telomere maintenance, cell cycle regulation, and DNA replication stress responses, underscoring its multifunctional nature.
Research has linked UPF1 dysregulation to human disease. Mutations or altered expression of UPF1 can disrupt RNA surveillance, contributing to neurodevelopmental disorders and cancer. For example, reduced NMD activity has been associated with enhanced tumor progression due to the stabilization of aberrant mRNAs. Conversely, hyperactive NMD can impair expression of regulatory transcripts, leading to developmental abnormalities. These findings highlight UPF1 as a key factor in both normal physiology and disease pathology.
At the molecular level, UPF1 contains an RNA helicase domain with ATP binding motifs and regulatory regions that mediate interactions with NMD cofactors. Phosphorylation of UPF1 by SMG1 kinase is a crucial step in triggering mRNA decay. Structural and biochemical studies have shown that UPF1 undergoes major conformational changes upon RNA binding and phosphorylation, enabling it to act as a dynamic regulator of RNA fate.
The UPF1 antibody is widely used in western blotting, immunohistochemistry, immunofluorescence, and flow cytometry to detect protein expression, localization, and phosphorylation status. These applications support research into RNA decay, transcriptional fidelity, and cancer biology. For investigators studying RNA metabolism, gene expression control, or surveillance pathways, the UPF1 antibody is a reliable and specific detection tool.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
