| Field | Specification |
|---|---|
| Mfr No | |
| Assay Time | |
| Detection Method | |
| Product Type | |
| Sample Type(s) | Urine |
| Shipping | |
| Species | |
| Storage |
Overview
An accurate, simple, fast (15 min) and inexpensive screening tool for the identification of protein putrefaction in the gastrointestinal tract. For research use only, not intended for diagnostic use. The Indican Reagent is corrosive. It is. The assay uses Obermeyer (Improved) for signal readout. Compatible sample input includes Urine. Typical stated assay timing is 15 min.
Key elements and design rationale
- Readout format: Obermeyer (Improved) supports plate-based signal acquisition and consistent comparison across matched samples.
- Sample compatibility: The stated sample scope includes Urine, which is useful when aligning matrix type with calibration and control design.
- Workflow timing: The listed assay time of 15 min helps frame batch planning, replicate handling, and plate throughput.
- Feature emphasis: Convenient. Only need to pipette 2 mL urine into the ready reagent vial, mix and read the indican level from a color chart.
Additional feature notes highlight Fast: 15 min. Available format information for this listing includes 20 Tests.
Biological background
This product is centered on measurement of urinary indican test within the matrices described for the assay. In practice, datasets from this type of format are typically interpreted by comparing relative signal, activity, or abundance across matched control and experimental groups rather than relying on a single value in isolation. Careful alignment of sample matrix, incubation window, and calibration strategy is important when comparing results across plates, operators, or study days.
More details
Indican (indoxyl sulfate) is a putrefaction product resulting from bacterial deconjugation of dietary tryptophan to indole in the small intestine. Indican levels are directly associated with bacterial activity in the intestines. Elevated levels indicate intestinal toxemia or overgrowth of anaerobic bacteria, putrefaction of undigested food in the bowels, stomach disorder (constipation, malabsorption), intestinal disorders, and pancreatic insufficiency.BioAssay Systems’ Urinary Indican Test Kit is a less hazardous modification of the original Obermeyer indican reagent. It provides an accurate, simple, fast (15 min), and inexpensive screening tool for the identification of protein putrefaction in the gastrointestinal tract.
Detection method
Colorimetric (Obermeyer (Improved)).
Procedures and timing
Stated procedure or timing information: 15 min.
Research relevance and current trends
- Plate-based quantification and side-by-side group comparison remain central use cases for this assay format.
- The product notes emphasize multi-sample throughput, making it relevant for screening-oriented and larger batch comparison studies.
- Short assay timing and plate compatibility support time-course or repeated-measure collection plans when handling is kept consistent.
Common research applications
- Quantify urinary indican test in urine by Obermeyer (Improved) readout.
- Compare treatment or phenotype groups using matched urine handling.
- Monitor time-course or pre/post changes in urine across study conditions.
Interpretation is usually strongest when signal changes are assessed alongside matrix-matched controls, replicate agreement, and the assay's stated analytical window.
Notes for experimental interpretation
- Matrix composition, background signal, and sample handling can influence apparent response; compare like-with-like whenever possible.
- Use appropriate blanks, controls, and replicate wells to distinguish biological differences from plate, reagent, or handling variability.
For laboratories requiring additional technical capacity, we provide scientific support services including assay execution, method guidance, product sourcing, and customization to align the assay with specific experimental objectives. If you need assistance selecting the appropriate kit configuration, adapting the workflow to your application, or identifying related research services, please click Talk to a Scientist, email support@biohippo.com, or review our Research Services; a member of our scientific team will follow up with recommendations tailored to your study.
