| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | V5 |
| Conjugate | |
| Formulation | |
| Host | |
| Immunogen | Synthetic peptide V5-Tag. |
| Product Type | |
| Reactivity | |
| Storage |
Product Overview
V5-tag mouse monoclonal antibody detects endogenous levels of internal, C-terminal, and N-terminal V5- tagged proteins.
The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids, although it has also been used with a shorter 9 amino acid sequence.
Validated Applications
| Application | Recommended Dilution |
|---|---|
| Western Blot (WB) | 1:5000~1:10000 |
| Immunofluorescence (IF) | 1:200 |
Product Specifications
| Clonality | Monoclonal |
|---|---|
| Host Species | Mouse |
| Isotype | IgG1 |
| Species Reactivity | All |
| Conjugate | Unconjugated |
| Purification | Affinity Purification |
| Formulation | Mouse IgG1, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 |
| Modification | Unmodified |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. |
Immunogen / Antigen
Synthetic peptide V5-Tag.
Safety & Handling
For Research Use Only (RUO). Not for diagnostic or therapeutic use. Handle as a potential biohazard in accordance with good laboratory practices.
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This antibody has been validated for WB, IF. Always refer to the product datasheet for recommended dilution ranges and appropriate positive control samples for each application type.
Validated reactivity has been confirmed for All. Cross-reactivity with additional species may exist based on sequence homology but has not been confirmed by the manufacturer. For unlisted species, we recommend running a preliminary validation experiment.
This antibody was raised in Mouse. For indirect detection (WB, IHC, IF), select an anti-Mouse secondary antibody that matches the isotype. For direct detection, check the variant selector for conjugated formats. Confirm secondary antibody compatibility before ordering.
Synthetic peptide V5-Tag.
Please refer to the storage conditions on the product label and datasheet. As a general best practice: upon receipt, aliquot into single-use volumes to minimize freeze-thaw cycling, which can progressively reduce antibody performance and lead to aggregation.
Validated reactivity covers All samples. Performance can vary across different cell lines and tissue types depending on target antigen expression levels and epitope accessibility. We recommend verifying target expression in your specific model system using a known positive control before proceeding to experimental samples.
BioHippo offers customization and value-added services for qualified research programs:
- Bulk Pricing: Volume discounts available for orders of 5 or more units. Request a quote through the Contact Us page.
- Custom Conjugation: Select antibody products are available for custom conjugation to fluorophores, biotin, HRP, or other detection labels upon request. Minimum quantities apply.
- Custom Formats: Lyophilized or concentrated formats may be available for select products. Inquire before ordering.
- Technical Support: Our scientific team is available to assist with application optimization, protocol troubleshooting, and experimental design.
For customization inquiries, please use the Contact Us page or reach out to our support team directly.
