| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | Amino acids HDQLAGSDDSEMED were used as the immunogen for this VGLUT1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
VGLUT1 Antibody / Vesicular glutamate transporter 1 is an antibody targeting VGLUT1, raised in Goat for protein detection and localization studies where these specifications are required.
Key elements and design rationale
- Target: VGLUT1.
- Antibody identity: Polyclonal (goat origin); Goat Ig.
- Conjugate/label: Unconjugated (affects detection chemistry and multiplex compatibility).
- Format: Antigen affinity purified.
- Species reactivity: Human.
- Listed applications: IHC-P, ELISA (peptide) (refer to on-page specifications for application-specific guidance).
Biological background
VGLUT1 (Vesicular glutamate transporter 1), also called SLC17A7 and BNPI (Brain-specific Na(+)-dependent inorganic phosphate cotransporter) is responsible for packaging glutamate, the most abundant neurotransmitter in the brain, into synaptic vesicles. These vesicles then release glutamate into the synapse, where it binds to receptors on the postsynaptic neuron, triggering a cascade of events that ultimately lead to the transmission of signals. Without VGLUT1, this process would be disrupted, leading to impaired neurotransmission and potentially affecting brain function. Research has shown that abnormalities in VGLUT1 expression are associated with various neurological disorders, including schizophrenia, Alzheimer's disease, and epilepsy.
Research relevance and current trends
- Comparative expression profiling across cell types, tissues, or perturbations (e.g., drug treatment, genetic editing, or differentiation).
- Subcellular localization and trafficking studies, including co-localization with pathway markers in microscopy-based assays.
- Integration of protein-level measurements with transcriptomics or proteomics to relate abundance to regulation and phenotype.
Common research applications
- Immunohistochemistry: researchers commonly compare relative signal levels across conditions and use appropriate negative/positive controls for interpretation.
- ELISA: researchers commonly compare relative signal levels across conditions and use appropriate negative/positive controls for interpretation.
Interpretation should account for antibody-dependent factors such as epitope accessibility, isoforms, and sample preparation differences across workflows.
Notes for experimental interpretation
- Isoforms and PTMs: many targets have multiple isoforms and post-translational modifications that can shift apparent signal or localization; interpret bands/signals accordingly.
- Epitope context: binding can depend on protein conformation and sample processing; region information in the title/immunogen can help anticipate what may be detected.
- Species differences: predicted or validated reactivity may vary by ortholog sequence and sample context; confirm in your model system.
- Control concepts: include negative controls (no-primary/isotype), and where possible genetic controls (KO/KD) or independent antibodies to strengthen conclusions.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
