| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthesized peptide derived from human WDR4 was used as the immunogen for the WDR4 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
WDR4 Antibody / WD repeat domain 4 is a anti-WDR4 Rabbit antibody Recombinant Rabbit Monoclonal clone 25W87 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS) with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: WDR4
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 25W87, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB, IHC, ICC, IF, FACS
Biological background
WDR4 is encoded by the WDR4 gene located on human chromosome 21q22.3. The protein is approximately 412 amino acids long and contains several WD40 repeats that mediate protein-protein interactions, forming a beta-propeller structure typical of the WD repeat family. WDR4 localizes to the nucleus and cytoplasm, where it partners with METTL1 to catalyze methylation of specific tRNA guanine residues, ensuring proper translation initiation and elongation.
The WDR4 antibody detects a 46 kilodalton protein by western blot and demonstrates nuclear and cytoplasmic staining patterns under immunofluorescence. Through its interaction with METTL1, WDR4 stabilizes the tRNA methyltransferase complex and regulates global protein synthesis. Loss of WDR4 leads to reduced tRNA methylation, impaired translation, and increased sensitivity to stress.
Mutations in WDR4 cause a spectrum of developmental and neurological disorders, including microcephaly and intellectual disability. These defects arise from disrupted tRNA modification, which affects the translation of growth-related and neuronal genes. In cancer, overexpression of WDR4 enhances translation of oncogenic proteins and promotes tumor progression through metabolic reprogramming.
Because of its key role in RNA modification and translation regulation, WDR4 serves as an essential model for understanding epitranscriptomic control of gene expression.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
